文献类型：期刊文献

英文题名：Colorimetric thrombin assay using aptamer-functionalized gold nanoparticles acting as a peroxidase mimetic

作者：Zhang, Laiping[1];Li, Li[1]

第一作者：张来苹

通讯作者：Zhang, LP[1]

机构：[1]Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China

第一机构：新乡学院化学化工学院

通讯机构：[1]corresponding author), Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China.|[110713]新乡学院化学化工学院;[11071]新乡学院;

年份：2016

卷号：183

期号：1

起止页码：485-490

外文期刊名：MICROCHIMICA ACTA

收录：;Scopus(收录号：2-s2.0-84953366970);WOS:【SCI-EXPANDED(收录号:WOS:000367692900055)】；

基金：All authors gratefully acknowledge the financial support of the Doctoral Program of Higher Education of China (1399020162).

语种：英文

外文关键词：Visual detection; Nanoparticles; Serum assay; Tetramethylbenzidine; Enzyme-like nanomaterial

摘要：The article describes a method for the determination of thrombin that is based on the use of gold nanoparticles (AuNPs) functionalized with an aptamer labeled with a horseradish peroxidase mimick. The assay is based on (a) the high affinity between aptamer and thrombin, and (b) on the finding that AuNPs display HRP-like properties in catalyzing the oxidation of 3,3,5,5-tetramethylbenzidine to give a blue reaction product with an absorption maximum at 652 nm. The limit of detection for thrombin is as low as 0.02 pM, and the analytical range extends from 0.1 pM to 0.1 mu M thrombin. The aptamer-based assay was successfully applied to the quantitation of thrombin in spiked human serum. This method is simple, inexpensive and highly sensitive.