文献类型：期刊文献

英文题名：The Characterization of SmedHSP90 Gene Using Methods of Bioinformatics

作者：Xi, Xingzi[1];Ma, Keshi

第一作者：席兴字

通讯作者：Xi, XZ[1]

机构：[1]Xinxiang Univ, Dept Educ Sci, Xinxiang 453003, Peoples R China

第一机构：新乡学院教育科学学院

通讯机构：[1]corresponding author), Xinxiang Univ, Dept Educ Sci, East Jinsui Rd, Xinxiang 453003, Peoples R China.|[1107119]新乡学院教育科学学院;[11071]新乡学院;

年份：2013

卷号：10

期号：1

起止页码：2836-2839

外文期刊名：LIFE SCIENCE JOURNAL-ACTA ZHENGZHOU UNIVERSITY OVERSEAS EDITION

收录：;Scopus(收录号：2-s2.0-84878974823);WOS:【SCI-EXPANDED(收录号:WOS:000322999200121)】；

语种：英文

外文关键词：Bioinformatics; HSP90; Planarian

摘要：Bioinformatics is a very powerful tool in the field of genomics study. Firstly, we obtained the heat shock protein 90 gene structure of planarian Schmitdea mediterranea (designated SmedHSP90) by using Smedgenome database. The ORF of SmedHSP90 is 2148 bp encoding a polypeptide of 715 amino acids with all five conserved motifs of HSP90 protein family signature. In addition, the gene structure of SmedHSP90 contains a 48 bp intron, and the 5'- and 3'- splicing site follows the typical "GT-AG" rule. Secondly, we constructed the HSP90 phylogenetic tree using the software Clustal 1.83 and Mega 3.1, and discussed the evolutionary position of SmedHSP90. Thirdly, three-dimensional domain structure of SmedHSP90 was predicted by SWISS-MODEL Server. Bioinformatic analysis of SmedHSP90 gene provides basic data for the study of stress response in planarians.