文献类型：期刊文献

英文题名：Characterization and Horizontal Transfer of Antimicrobial Resistance Genes and Integrons in Bacteria Isolated from Cooked Meat Products in China

作者：Yu, Tao[1];Jiang, Xiaobing[3];Liang, Yu[2];Zhu, Yanping[1];Tian, Jinhe[1];Ying, Hao[1];Wang, Xuannian[1];Shi, Lei[4]

第一作者：于涛

通讯作者：Jiang, XB[1]

机构：[1]Xinxiang Univ, Coll Life Sci & Technol, Xinxiang 453003, Peoples R China;[2]Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China;[3]Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China;[4]Jinan Univ, Inst Food Safety & Nutr, Guangzhou 510632, Guangdong, Peoples R China

第一机构：新乡学院生命科学技术学院

通讯机构：[1]corresponding author), Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China.

年份：2017

卷号：80

期号：12

起止页码：2048-2055

外文期刊名：JOURNAL OF FOOD PROTECTION

收录：;Scopus(收录号：2-s2.0-85053189394);WOS:【SCI-EXPANDED(收录号:WOS:000417726400012)】；

基金：This work was supported by the Key Project of Natural Science of the Education Department of Henan Province, China (15A180006 and 16A180011), the Key Scientific and Technological Project of Xinxiang (ZG15007, ZG13012), the Foundation for Youths of Henan Normal University, and the Doctoral Scientific Research Foundation of Henan Normal University.

语种：英文

外文关键词：Antimicrobial resistance; Cooked meat products; Integron; Resistance gene

摘要：The aim of this study was to investigate antimicrobial resistance and the presence and transferability of corresponding resistance genes and integrons in bacteria isolated from cooked meat samples in the People's Republic of China. A total of 150 isolates (22 species belonging to 15 genera) were isolated from 49 samples. Resistance of these isolates to antimicrobials was commonly observed; 42.7, 36.0, and 25.3% of the isolates were resistant to tetracycline, streptomycin, and ampicillin, respectively. Multidrug resistance was observed in 41 (27.3%) of the isolates. Sixteen resistance genes, i.e., bla(TEM-1) and bla(CTX-M-14) (beta-lactams), aac(3)-IIa (gentamicin), strA and strB (streptomycin), qnrB and qnrS (fluoroquinolone), sul1, sul2, and sul3 (sulfamethoxazole), cat1 and cat2 (chloramphenicol), and tetM, tetA, tetS, and tetB (tetracycline), were found in 54 isolates. One isolate of Pseudomonas putida carried qnrB, and sequence analysis of the PCR product revealed 96% identity to qnrB2. The qnr genes were found coresiding and were cotransferred with bla genes in two isolates. Twelve isolates were positive for the class 1 integrase gene, and four isolates carried the class 2 integrase gene. However, no class 3 integrase gene was detected. One isolate of Proteus mirabilis carried dfrA32-ereA-aadA2, and this unusual array could be transferred to Escherichia coli. Nonclassic class 1 integrons lacking qacE Delta 1 and sul1 genes were found in 2 of the 12 intI1-positive isolates. Our results revealed the presence of multidrug-resistant bacteria in cooked meats and the presence and transferability of resistance genes in some isolates, suggesting that cooked meat products may act as reservoirs of drug-resistant bacteria and may facilitate the spread of resistance genes.