文献类型：期刊文献

英文题名：SMALL-SCALE ISOLATION OF MEIOCYTES FROM ARABIDOPSIS THALIANA ANTHERS FOR CYTOLOGICAL ANALYSES

作者：Li, Junhua[1,3];Yuan, Jinhong[1,3];Ji, Kang[1];Zhang, Yanling[2];Wang, Juan[1];Zhang, Shasha[1];Liu, Shubo[1];Qiu, Pengfei[1];Li, Ning[1];Li, Mingjun[1,3]

第一作者：Li, Junhua

通讯作者：Li, JH[1];Li, MJ[1];Li, JH[2];Li, MJ[2]

机构：[1]Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China;[2]Xinxiang Univ, Sch Chem & Chem Engn, Xinxiang 453003, Peoples R China;[3]Engn Lab Green Med Mat Biotechnol, Xinxiang 453007, Henan Province, Peoples R China

第一机构：Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China

通讯机构：[1]corresponding author), Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China;[2]corresponding author), Engn Lab Green Med Mat Biotechnol, Xinxiang 453007, Henan Province, Peoples R China.

年份：2016

卷号：48

期号：5

起止页码：1989-1992

外文期刊名：PAKISTAN JOURNAL OF BOTANY

收录：;Scopus(收录号：2-s2.0-84994189458);WOS:【SCI-EXPANDED(收录号:WOS:000392910800026)】；

基金：This work was supported by the National Natural Science Foundation of China (31670317), the Scientific Research Foundation for Returned Oversea Scholars, Ministry of Education of China, the Joint Talent Cultivation Funds of NSFC-HN (U1504319), and the Key Research Fund of Higher Education of Henan Province (15A180044).

语种：英文

外文关键词：Arabidopsis thaliana; Callose; Chromosomes; Pollen mother cells; Meiosis; Reporter assays

摘要：In flowering plants, the meiocytes develop within nourishing tissues the anthers or the ovaries. Although most plants bear one or more anthers in each bisexual or male flower, the size of anthers that undergo meiosis is relatively small, and each anther contains only a small fraction of male meiocytes. As a consequence, the isolation of plant meiocytes is very challenging due to their relative inaccessibility. Efficient manual dissection of meiocytes from young Arabidopsis anthers for cytological and cytogenetic analyses is technically challenging. Indirect detection of cellular or nuclear phenotypes is impaired by poor penetration of the reagents, and cell type-specific imaging is deminished by the lack of clarity in whole mount tissues. Here, we describe a method that allows the efficient isolation of male meiocytes of Arabidopsis. Anthers undergoing meiosis were dissected and collected into appropriate isolation/labeling buffer, depending on the downstream application. Meiocytes were released by crushing with a cover slip on a glass slide. The isolated meiocytes could be analyzed using a microscope. The technical skills required are simple, and the procedure does not require any special equipment. Isolated meiocytes are suitable for a variety of dowstream applications, such as cell morphological investigation, reporter gene assays, chromosome analyses, and callose staining.