文献类型：期刊文献

英文题名：Contribution of prgH gene for Salmonella Pullorum to virulence and the expression of NLRP3, Caspase-1 and IL-1β in chickens

机构：[1]Medical College, Xinxiang University, Xinxiang, China;[2]School of Life Science and Basic Medicine, Xinxiang University, Xinxiang, China

第一机构：新乡学院医学院

年份：2022

卷号：171

外文期刊名：Microbial Pathogenesis

收录：Scopus(收录号：2-s2.0-85137646482)

基金：National Natural Science Foundation of China ( 81702074 ); Natural Science Foundation of Henan ( 202300410014 ); and PhD Programs Foundation of Xinxiang University ( 1366020121 ).

语种：英文

外文关键词：Caspase-1; IL-1β; NLRP3; prgH; Sallmonella pullorum; Virulence

摘要：Type III secretion system 1 (T3SS1) encoded by Salmonella pathogenicity island 1 (SPI1) is associated with invasion of host cells by Salmonella, PrgH encoded by prgH gene is an important component of T3SS1. This study aimed to explore the contribution of prgH gene for Salmonella Pullorum to virulence and the expression of NLRP3, Caspase-1 and IL-1β in chickens. A prgH gene deletion mutant (C79-13ΔprgH) was firstly generated, and the result of LD50 showed that deletion of prgH significantly decreased the virulence of Salmonella Pullorum in one-day-old HY-line white chickens, and the colonization also decreased in chickens after loss of prgH. Next, the expressions of NLRP3, Caspase-1, and IL-1β were detected in acute infection model of chickens by qRT-PCR and/or ELISA, respectively, and the results showed that the mutant strain C79-13ΔprgH reduced the expression levels of NLRP3, Caspase-1, and IL-1β in chickens compared to the group infected with the wild type strain C79-13. Taken together, all of these findings indicated that prgH promotes the virulence and the expression of NLRP3, Caspase-1, and IL-1β for Salmonella Pullorum in chickens. ? 2022 Elsevier Ltd