文献类型：期刊文献

英文题名：A Coumarin-Based Fluorescence Probe for Selective Recognition of Cu2+ Ions and Live Cell Imaging

作者：He, Guangjie[1];Ma, Nana[2];Li, Linlin[1];Xie, Chenyan[1];Yang, Linlin[1];Xu, Jinhe[1];Wang, Junli[3]

第一作者：He, Guangjie

通讯作者：He, GJ[1];Yang, LL[1];Wang, JL[2]

机构：[1]Xinxiang Med Univ, Sch Forens Med, Xinxiang Key Lab Forens Sci Evidence, Jinsui Rd 601, Xinxiang 453003, Henan, Peoples R China;[2]Henan Normal Univ, Henan Key Lab Boron Chem & Adv Energy Mat, Henan Key Lab Organ Funct Mol & Drug Innovat, Sch Chem & Chem Engn, East Construct Rd 46, Xinxiang 453007, Henan, Peoples R China;[3]Xinxiang Univ, Coll Chem & Chem Engn, Jinsui Rd 191, Xinxiang 453003, Henan, Peoples R China

第一机构：Xinxiang Med Univ, Sch Forens Med, Xinxiang Key Lab Forens Sci Evidence, Jinsui Rd 601, Xinxiang 453003, Henan, Peoples R China

通讯机构：[1]corresponding author), Xinxiang Med Univ, Sch Forens Med, Xinxiang Key Lab Forens Sci Evidence, Jinsui Rd 601, Xinxiang 453003, Henan, Peoples R China;[2]corresponding author), Xinxiang Univ, Coll Chem & Chem Engn, Jinsui Rd 191, Xinxiang 453003, Henan, Peoples R China.|[110713]新乡学院化学化工学院;[11071]新乡学院;

年份：2019

卷号：2019

外文期刊名：JOURNAL OF SENSORS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000496994300001)】；

基金：We gratefully acknowledge the financial support from the National Natural Science Foundation of China (Nos. 21801215 and 21371148), the Natural Science Foundation of Henan Province (No. 182300410309), the Key Scientific and Technological Project of Henan Province (No. 182102310648), the Key Research Project of Higher Education Institutions of Henan Province (No. 18A150044), and the National College Students' Innovation and Entrepreneurship Training Program (No. 201610472020).

语种：英文

外文关键词：Absorption spectroscopy - Emission spectroscopy - Fluorescence - Metal ions - Metals - Probes - Ultraviolet spectroscopy

摘要：A new fluorescence probe L was rationally designed and synthesized for the recognition of Cu2+ ions by the combination of coumarin hydrazide and 2-acetylpyrazine. The photochemical properties and selectivity of L for Cu2+ ions in a CH3CN/HEPES (3:2, v/v) buffer were investigated by UV-vis absorption and fluorescence emission spectra. A highly selective and sensitive response of L for Cu2+ ions over other competing metal ions was observed with limit of detection in 3 mu M. The coordination stoichiometry of L to Cu2+ ions was determined to be 1:1 by the UV-vis absorption spectrum, the fluorescence titrations, and density functional theory (DFT) calculations. Moreover, L was applied successfully for recognition of intracellular Cu2+ ions in living cells.