文献类型：期刊文献

英文题名：Quantitative Determination of Nitrofurazone Metabolites in Animal-Derived Foods Based on a Background Fluorescence Quenching Immunochromatographic Assay

作者：Wu, Yuping[1];Wang, Jia[2];Zhou, Yong[3];Qi, Yonghua[1];Ma, Licai[4];Wang, Xuannian[1];Tao, Xiaoqi[2]

通讯作者：Wang, XNA[1];Tao, XQ[2]

机构：[1]Xinxiang Univ, Coll Life Sci & Basic Med, Xinxiang 453003, Henan, Peoples R China;[2]Southwest Univ, Coll Food Sci, Chongqing 400715, Peoples R China;[3]China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China;[4]Beijing WDWK Biotech Co Ltd, Beijing 100095, Peoples R China

第一机构：新乡学院

通讯机构：[1]corresponding author), Xinxiang Univ, Coll Life Sci & Basic Med, Xinxiang 453003, Henan, Peoples R China;[2]corresponding author), Southwest Univ, Coll Food Sci, Chongqing 400715, Peoples R China.|[11071]新乡学院;

年份：2021

卷号：10

期号：7

外文期刊名：FOODS

收录：;Scopus(收录号：2-s2.0-85111411336);WOS:【SCI-EXPANDED(收录号:WOS:000676208800001)】；

基金：This research was funded by the National Key Research and Development Program of China (2018YFC1602900), Key Scientific Research Projects of Higher Education in Henan Province (19B230015), Doctoral Research Initiation Fund of Xinxiang University (1366020122), Henan Scientific and Technological Project (212102310909).

语种：英文

外文关键词：semicarbazide (SEM); background fluorescence-quenching immunochromatographic assay (bFQICA); quantitative determination; animal-derived foods

摘要：Due to their facile synthesis and friendly functionalization, gold nanoparticles (AuNPs) have been applied in all kinds of biosensors. More importantly, these biosensors, with the combination of AuNPs and immunoassay, are expected to be used for the detection of different compounds with low concentrations in complex samples. In this study, a AuNPs-labeled antibody immunoprobe was prepared and combined with a fluorescence-quenching principle and a background fluorescence-quenching immunochromatographic assay (bFQICA), achieving rapid on-site detection. By using a portable fluorescence immunoquantitative analyzer and a QR code with a built-in standard curve, the rapid quantitative determination for nitrofurazone metabolite of semicarbazide (SEM) in animal-derived foods was realized. The limits of detection (LODs) for bFQICA in egg, chicken, fish, and shrimp were 0.09, 0.10, 0.12, and 0.15 mu g kg(-1) for SEM, respectively, with the linear range of 0.08-0.41 mu g L-1, the recoveries ranging from 73.5% to 109.2%, and the coefficient of variation <15%, only taking 13 min for the SEM detection. The analysis of animal-derived foods by bFQICA complied with that of liquid chromatography-tandem mass spectrometry (LC-MS/MS).