文献类型：期刊文献

英文题名：In situamplified photothermal immunoassay for neuron-specific enolase with enhanced sensitivity using Prussian blue nanoparticle-loaded liposomes

作者：Zhi, Li-Juan[1,2];Sun, Ai-Li[1];Tang, Dianping[2]

第一作者：职丽娟;Zhi, Li-Juan

通讯作者：Sun, AL[1];Tang, DP[2]

机构：[1]Xinxiang Univ, Dept Chem & Chem Engn, Xinxiang 453000, Henan, Peoples R China;[2]Fuzhou Univ, Dept Chem, Key Lab Analyt Sci Food Safety & Biol, MOE & Fujian Prov, Fuzhou 350108, Peoples R China

第一机构：新乡学院化学化工学院

通讯机构：[1]corresponding author), Xinxiang Univ, Dept Chem & Chem Engn, Xinxiang 453000, Henan, Peoples R China;[2]corresponding author), Fuzhou Univ, Dept Chem, Key Lab Analyt Sci Food Safety & Biol, MOE & Fujian Prov, Fuzhou 350108, Peoples R China.|[110713]新乡学院化学化工学院;[11071]新乡学院;

年份：2020

卷号：145

期号：12

起止页码：4164-4172

外文期刊名：ANALYST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000542708600007)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Grant No.: 51802275), the Key Scientific and Technological Research Project of Henan Province (Grant No.: 202102310298), the Research Fund for the Technological Innovative Scholar of Xinxiang University (China), and the Henan Photoelectrocatalytic Material and Micro-nano Application Technology Academician Workstation, Xinxiang, Henan 450003, China.

语种：英文

外文关键词：Antibodies - Infrared devices - Liposomes - Nanoparticles - Synthesis (chemical) - Thermometers

摘要：Methods based on prussian blue nanoparticles (PBNPs) have been reported for photothermal immunoassays in analytical nanoscience fields but most suffer from low sensitivity and are not beneficial for routine use. Herein, we design anin situamplified near-infrared (NIR) photothermal immunoassay for the quantitative screening of neuron-specific enolase (NSE) on a portable thermometer using PBNP-encapsulated nanoliposomes as photosensitive materials. Biotinylated liposomes loaded with numerous prussian blue nanoparticles were synthesized through a typical reverse-phase evaporation method. The photothermal immunoassay was carried out in an anti-NSE capture antibody-coated microplate using the biotinylated anti-NSE secondary antibody. With the sandwiched immunoreaction and the biotin-avidin linkage, the subsequent photothermal measurement of PBNPs released from the liposomes with buffered surfactant including Tween 20 was conducted on a digital thermometer under near-infrared 808 nm laser irradiation, accompanied by the convertion of NIR-light wavelength to heat. Under the optimum conditions, the photothermal immunoassay displayed a wide dynamic concentration range of 0.1-100 ng mL(-1)with a low detection limit for NSE of 0.053 ng mL(-1). Good reproducibility (RSD <= 2.78% for intra-assay; RSD <= 4.39% for inter-assay), high selectivity against other biomarkers, and a long-term stability (>= 94.9% of the initial signal during six-month storage) were acquired in the photothermal immunoassay. Impressively, the analysis of 7 human serum specimens for target NESviathe photothermal immunoassay also gave well-matched results with the referenced human NSE enzyme-linked immunosorbent assay.