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西瓜中类胡萝卜素的分离与分析    

Separation and Analysis of Carotenoids in Watermelon Flesh

文献类型:期刊文献

中文题名:西瓜中类胡萝卜素的分离与分析

英文题名:Separation and Analysis of Carotenoids in Watermelon Flesh

作者:胡利强[1];郭晓伟[1];赵文恩[2];王军伟[3];张广瑞[3]

第一作者:胡利强

机构:[1]新乡学院化学与化工学院;[2]郑州大学化学工程与能源学院;[3]河南心连心化肥有限公司

第一机构:新乡学院化学化工学院

年份:2011

卷号:40

期号:9

起止页码:150-156

中文期刊名:河南农业科学

外文期刊名:Journal of Henan Agricultural Sciences

收录:CSTPCD;;北大核心:【北大核心2008】;CSCD:【CSCD_E2011_2012】;

语种:中文

中文关键词:西瓜;类胡萝卜素;高效液相色谱法

外文关键词:Watermelon; Caroternoids; HPLC

摘要:采用超声波辅助提取法从不同瓤色西瓜果肉中提取类胡萝卜素,经柱色谱分离和重结晶纯化,获得纯度为90%左右的番茄红素、β-胡萝卜素和ζ-胡萝卜素,用作分析的标样。采用高效液相色谱法,通过特征吸收光谱和保留时间鉴别,初步分析西瓜中类胡萝卜素的组成。结果表明,红瓤西瓜中的类胡萝卜素以番茄红素(18.55%)和β-胡萝卜素为主(11.71%);黄瓤西瓜中含较多新黄质(52.02%);白瓤西瓜中不含番茄红素。
Carotenoids were extracted from the fruit flesh of five watermelon varieties using ultrasonic method,and separated by open-column chromatography using recryctallization. Lycopene,β- carotene,and k-carotene in 90% purity were obtained and used in analysis of watermelon carotenoids as the standard samples. Watermelon carotenoids were analyzed by high performance liquid chromatography with the photodiode array detector. The results showed that lycopene (18.55 %) and 13-carotene (11.71%) were the major pigments in the red-fleshed watermelon. In the yellowfleshed watermelon,the neoxanthin content(52.02%) was relatively high. Lycopene was not detected in the white-fleshed wtaremelon.

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