文献类型：期刊文献

英文题名：Infectivity and propagation of attenuated infectious bursal disease virus in the chicken B-lymphocyte cell line DT40

作者：Luo, Jun[1];Zhang, Gai-Ping[1];Fan, Jian-Ming[1,2];Teng, Man[1];You, Lei-Ming[1];Zhou, Ling[1];Deng, Rui-Guang[1];Wang, Xuan-Nian[1,3];Yang, Yan-Yan[1];Wang, Li[1];Xing, Guang-Xu[1];Cheng, Na[1]

第一作者：Luo, Jun

通讯作者：Zhang, GP[1]

机构：[1]Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China;[2]Zhengzhou Univ, Coll Publ Hlth, Toxicol Lab, Zhengzhou 450001, Peoples R China;[3]Xinxiang Univ, Xinxiang 453003, Peoples R China

第一机构：Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China

通讯机构：[1]corresponding author), Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China.

年份：2009

卷号：154

期号：3

起止页码：513-517

外文期刊名：ARCHIVES OF VIROLOGY

收录：;Scopus(收录号：2-s2.0-62149133840);WOS:【SCI-EXPANDED(收录号:WOS:000263915400016)】；

基金：This work was supported by the National Basic Research Program (2005CB523200), National Key Technology R&D Program (2006BAD06A04-6) and Natural Science Foundation of Henan Province (082300433201). The authors also gratefully acknowledge the critical review of the manuscript by Dr. Avraham Rasooly (Division of Cancer Treatment and Diagnosis, National Cancer Institute, National Institutes of Health, USA).

语种：英文

摘要：In this paper, the infectivity and propagation of two attenuated infectious bursal disease virus (IBDV) strains in DT40 cells were investigated. The results showed that both of the tested strains, TAD and HN3, directly infect and proliferate in DT40 cells, requiring no adaptive passages. Unexpectedly, IBDV can be rapidly propagated and continuously harvested at high titers for a long time, accompanied by the rapid growth of host cells and showing no increase in pathogenicity. Our results provide further support to suggest that DT40 cells can be used as an ideal model for studying IBDV pathogenesis. Additionally, the DT40 cell line could also serve as a potential system for commercial IBDV vaccine preparation.