文献类型：期刊文献

中文题名：Anti-tumor Effect of Newcastle Disease Virus Expressing IL-2 in Lung Cancer Model

作者：Li De-shan[1];Jiang Shan[1];Liu Yun-ye[1];Rasoul Lubna[1,2];He Jin-jiao[3];Mir Hassan Khoso[1];Cao Hong-xue[1];Yang Jiarui[1];Yin He[1];Yin Jie-chao[1]

第一作者：Li De-shan

机构：[1]College of Life Sciences,Northeast Agricultural University,Harbin 150030,China;[2]College of Science,University of Baghdad,Baghdad,Iraq;[3]College of Life Sciences,Xinxiang University,Xinxiang 453000,Henan,China

第一机构：College of Life Sciences,Northeast Agricultural University,Harbin 150030,China

年份：2020

卷号：27

期号：2

起止页码：42-50

中文期刊名：东北农业大学学报：英文版

基金：Supported by the National Key R&D Program of China(2017YFD0501102);the National Natural Science Foundation of China(31200121)。

语种：英文

中文关键词：IL-2;lung cancer;LLC cell line;rNDV;reverse genetic technique

摘要：Recombinant Newcastle disease virus(rNDV)has shown an anti-cancer effect in preclinical studies,but has never been tested for lung cancer models.This study explored the anti-cancer activity of genetically modified NDV expressing IL-2(rNDV–IL-2)in lung cancer models.This study used Lewis lung carcinoma cell line(LLC)to create tumor models in C57 female mice,the tumor-bearing mice were treated with rNDV-IL-2,rNDV and phosphate-buffered saline(PBS),respectively.In vitro results revealed that rNDV effectively infected malignant cells and expressed IL-2,in vivo results revealed that rNDV expressing IL-2 was highly efficient in inhibiting lung cancer tumors,with an average tumor size of 291.255 mm^3 in rNDV-IL-2 group compared to 763.068 mm^3 in rNDV group and 1101.68 mm^3 in PBS group.For the survival studies,treatment with rNDV-IL-2 enhanced the survival rates of tumor-bearing mice by 36%compared to those of rNDV treated mice and by 80%compared to those of vehicle-treated mice(survival rate:12 out of 15 for rNDV-IL-2 group;seven out of 15 for rNDV group and zero out of 15 for vehicle group).These results demonstrated that rNDV-expressed IL-2 enhanced the intrinsic anti-tumor ability of Newcastle disease virus in lung cancer models by further restrain of lung tumor growth and improvement of the survival rates of the tumor-bearing mice.The genetically modified rNDV-IL-2 was a good candidate for lung cancer therapy.