文献类型：期刊文献

英文题名：Salmonella Pullorum lacking srfA is attenuated, immunogenic and protective in chickens

作者：Yin, Junlei[1];Xiong, Wenhui[1];Yuan, Xinzhong[1];Li, Shuli[1];Zhi, Lijuan[1];Pan, Pengtao[1];Sun, Weiwei[1];Yu, Tao[2];He, Qunli[3,4];Cheng, Zhao[2]

通讯作者：Cheng, Z[1];He, QL[2]

机构：[1]Xinxiang Univ, Med Coll, Xinxiang, Henan, Peoples R China;[2]Xinxiang Univ, Sch Life Sci & Technol, Xinxiang, Henan, Peoples R China;[3]Zhengzhou Univ Ind Technol, Med Coll, 16 Xueyuan Rd, Zhengzhou 451100, Peoples R China;[4]Xinxiang Med Univ, Coll Basic Med, Xinxiang, Henan, Peoples R China

第一机构：新乡学院

通讯机构：[1]corresponding author), Xinxiang Univ, Sch Life Sci & Technol, Xinxiang, Henan, Peoples R China;[2]corresponding author), Zhengzhou Univ Ind Technol, Med Coll, 16 Xueyuan Rd, Zhengzhou 451100, Peoples R China.|[1107115]新乡学院生命科学技术学院;[11071]新乡学院;

年份：2021

卷号：161

外文期刊名：MICROBIAL PATHOGENESIS

收录：;Scopus(收录号：2-s2.0-85116664781);WOS:【SCI-EXPANDED(收录号:WOS:000710030200009)】；

基金：National Natural Science Foundation of China ( 81702074 ); Natural Science Foundation of Henan ( 202300410014 ); Henan of Programs for Science and Technology Development ( 212102110160 ); and PhD Programs Foundation of Xinxiang University ( 1366020121 ).

语种：英文

外文关键词：Sallmonella pullorum; srfA; Vaccine; Chicken; Protective efficacy

摘要：Sallmonella Pullorum is a host-restricted pathogen for poultry and causes severe economic importance in many developing countries. The development of novel vaccines for Salmonella Pullorum is necessary to eradicate the prevalence of the pathogen. In our study, a srfA deletion mutant (C79-13 Delta srfA) of Salmonella Pullorum was constructed, and then the biological characteristics and protective efficacy of the mutant were evaluated. The mutant C79-13 Delta srfA was much less virulent than its parental strain C79-13 in one-day-old HY-line white chickens, immunization with C79-13 Delta srfA (4 x 107 CFU) through oral pathway induced highly specific humoral and cellular immune responses, the growth performance of vaccinated chickens was consistent with that of unvaccinated chickens. The survival percentages of vaccinated chickens reached 90% and 80%, after challenge with Salmonella Pullorum strain C79-13 and Salmonella Gallinarum strain SG9 at 10 days post-immunization (dpi), respectively. Collectively, our results indicate that C79-13 Delta srfA is a live attenuated vaccine candidate.