文献类型：期刊文献

中文题名：冬小麦成熟胚离体培养中的消毒处理研究

英文题名：Sterilization Treatments of Triticum aetivum Mature Embryo Culture in vitro

作者：杨玉萍[1];李红玲[1];应浩[1]

第一作者：杨玉萍

机构：[1]新乡学院化学与化工学院

第一机构：新乡学院化学化工学院

年份：2010

期号：15

起止页码：7764-7766

中文期刊名：安徽农业科学

外文期刊名：Journal of Anhui Agricultural Sciences

收录：北大核心:【北大核心2008】；

语种：中文

中文关键词：小麦;种胚;组织培养;消毒处理

外文关键词：Triticum aetivum; Embryo; Tissue culture; Sterilization

摘要：[目的]研究小麦成熟胚离体培养时的消毒处理。[方法]采用复合、单独使用消毒剂的消毒方法,对6个冬小麦品种进行消毒。[结果]高浓度升汞对种胚造成伤害,品种间有遗传差异。[结论]复合使用消毒剂处理的愈伤组织质量明显好于单独使用的,一定浓度的次氯酸钠对种胚愈伤组织诱导有促进作用;各个品种最佳处理不同,75%酒精1min+12%次氯酸钠8min+0.10%升汞12～18min效果比较好;利福平、多菌灵处理对小麦的种胚消毒起到一定作用,但出愈率有所下降。
[Objective] The research aimed to study sterilization treatments of Triticum aetivum mature embryo culture in vitro.[Method] The experiments were carried out by using compound and single disinfectants to six varieties of Triticum aetivum.[Result] High concentrations of mercuric chloride made damage to embryo,and there were genetic differences between breeds.[Conclusion] Quality of callus treated by using compound disinfectants showed better than single disinfectant obviously.A certain concentration of sodium hypochlorite could promote embryo callus induction.The best treatments to varieties were different.75% alcohol 1 min ＋12%NaClO 8 min ＋0.1% mercuric chloride 12 to 18 minutes was the best.The treatment by rifampicin,carbendazim played a certain role on disinfection of Triticum aetivum embryo,but callus formation declined.