文献类型：期刊文献

英文题名：Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test

作者：Jin, Q. Y.[1];Feng, L. L.[2];Wang, Y. B.[3];Li, P.[4];Yang, J. F.[1];Teng, M.[1];Chai, S. J.[1];Xing, G. X.[1];Zhang, G. P.[1]

第一作者：Jin, Q. Y.

通讯作者：Zhang, GP[1]

机构：[1]Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China;[2]Henan Acad Agr Sci, Inst Agr Econ & Informat, Zhengzhou 450002, Peoples R China;[3]Xinxiang Med Univ, Sch Publ Hlth, Xinxiang 453003, Henan, Peoples R China;[4]Xinxiang Univ, Sch Life Sci & Basic Med, Xinxiang 453003, Henan, Peoples R China

第一机构：Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

通讯机构：[1]corresponding author), Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China.

年份：2022

卷号：25

期号：1

起止页码：27-34

外文期刊名：POLISH JOURNAL OF VETERINARY SCIENCES

收录：;Scopus(收录号：2-s2.0-85132318401);WOS:【SCI-EXPANDED(收录号:WOS:000779784000004)】；

基金：This work was supported by grants from the Key Scientific and Technological Research Pro-jects of Henan Province (No. 202102110100, No. 212102310335 and No. 202102110094) and the Key Scientific and Technological Research Projects of Xinxiang City (No. GG2019018).

语种：英文

外文关键词：porcine circovirus type 2 (PCV2); screening of MAbs; paper test

摘要：A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.