文献类型：期刊文献

英文题名：Role of Efflux Pumps in the in vitro Development of Ciprofloxacin Resistance in Listeria monocytogenes

作者：Jiang, Xiaobing[1];Yu, Tao[2];Xu, Ping[2];Xu, Xiaobo[2];Ji, Shengdong[1];Gao, Wujun[1];Shi, Lei[3]

第一作者：Jiang, Xiaobing

通讯作者：Yu, T[1]

机构：[1]Henan Normal Univ, Coll Life Sci, Xinxiang, Peoples R China;[2]Xinxiang Univ, Coll Life Sci & Technol, Xinxiang, Peoples R China;[3]Jinan Univ, Inst Food Safety & Nutr, Guangzhou, Guangdong, Peoples R China

第一机构：Henan Normal Univ, Coll Life Sci, Xinxiang, Peoples R China

通讯机构：[1]corresponding author), Xinxiang Univ, Coll Life Sci & Technol, Xinxiang, Peoples R China.|[1107115]新乡学院生命科学技术学院;[11071]新乡学院;

年份：2018

卷号：9

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85064191346);WOS:【SCI-EXPANDED(收录号:WOS:000445753600001)】；

基金：This work was supported by the National Natural Science Foundation of China (31601568), the Key Project of Natural Science of the Education Department of Henan Province, China (16A180011), the Program for Innovative Research Team in University of Henan Province (17IRTSTHN017), and the Project of Modern Agricultural Technology System of Henan Province (Z2012-04-02).

语种：英文

外文关键词：Listeria monocytogenes; efflux pump; Lde; ciprofloxacin; resistance

摘要：Efflux is a primary fluoroquinolone resistance mechanism in Listeria monocytogenes. In the present study, ciprofloxacin resistant strains were selected by exposure of sensitive strain to progressively increasing concentrations of ciprofloxacin and then the roles of efflux pumps Lde and MdrL in the development of resistance to ciprofloxacin were also investigated in L. monocytogenes. Ciprofloxacin sensitive strain of L. monocytogenes exhibited reduced susceptibility to this antibiotic after induction. Cross-resistance to ethidium bromide (EtBr) was observed in ciprofloxacin-induced strains. However, cross-resistance to benzalkonium chloride (BC) did not occur in this study. Compared to the wild-type strain HL06, the expression levels of lde were increased in four ciprofloxacin-induced strains. The single-gene deletion mutants of lde and mdrL from the ciprofloxacin-induced resistant strain HL06CIP4 were constructed. However, decreased minimum inhibitory concentration (MIC) of ciprofloxacin was observed only in HL06CIP4 Delta lde compared to that of the parental strain HL06CIP4. Ciprofloxacin uptake appeared to be obviously increased in HL06CIP4 Delta lde in relative to HL06CIP4. These evidences suggested that efflux pump Lde is involved in ciprofloxacin resistance in L. monocytogenes HL06CIP4. The deletion of lexA had no effect on the expression levels of lde in HL06CIP4 in the absence or presence of ciprofloxacin, indicating that LexA was not involved in the regulation of efflux pump Lde in L. monocytogenes.