文献类型：期刊文献

英文题名：The sug operon involves in resistance to quaternary ammonium compounds in Listeria monocytogenes EGD-e

作者：Jiang, Xiaobing[1]; Ren, Siyu[1]; Geng, Yimin[1]; Yu, Tao[2]; Li, Yi[1]; Liu, Lei[1]; Liu, Guosheng[1]; Wang, Hailei[1]; Shi, Lei[3]

第一作者：Jiang, Xiaobing

通讯作者：Wang, Hailei

机构：[1] College of Life Sciences, Henan Normal University, Xinxiang, China; [2] College of Life Science and Technology, Xinxiang University, Xinxiang, China; [3] Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China

第一机构：College of Life Sciences, Henan Normal University, Xinxiang, China

通讯机构：[1]College of Life Sciences, Henan Normal University, Xinxiang, China;[2]College of Life Science and Technology, Xinxiang University, Xinxiang, China|[1107115]新乡学院生命科学技术学院;[11071]新乡学院;

年份：2020

卷号：104

期号：16

起止页码：7093-7104

外文期刊名：Applied Microbiology and Biotechnology

收录：EI(收录号：20202608872543);Scopus(收录号：2-s2.0-85087080387)

语种：英文

外文关键词：Diseases - Antimicrobial agents - Electrophoretic mobility - Listeria - Chlorine compounds - Cationic surfactants

摘要：In recent years, an increasing number of Listeria monocytogenes strains with resistance to quaternary ammonium compounds (QACs) have been reported. However, the genetic basis for QACs resistance in L. monocytogenes remains poorly understood. In the present study, we have characterized the operon lmo0852/lmo0853/lmo0854 (designated sugR/sugE1/sugE2) that contributes to QACs' resistance in L. monocytogenes EGD-e. We constructed the gene deletion mutants and the complemented strains, determined minimum inhibitory concentrations (MICs) of these strains against antimicrobial agents, assessed the transcription levels of target genes by RT-qPCR, and measured the promoter activity by using β-galactosidase assays. We also investigated the interaction between the promoter DNA and the putative regulatory protein by electrophoretic mobility shift assay (EMSA). The sug operon consists of a putative TetR family regulator encoded by sugR and two small multidrug resistance (SMR) efflux pumps encoded by sugE1 and sugE2. Our results showed that either SugE1 or SugE2 is sufficient for QACs' resistance, indicating their function overlapping in QACs' resistance. Interestingly, lacking one sugE gene could lead to a significant increase in transcription of the other sugE gene in the presence of benzalkonium chloride (BC). Additionally, SugR negatively regulates the transcription of the sug locus by binding to the operon promoter. Given that QACs are commonly used in food industry, the findings from this study will help us to have a better understanding of the adaptations of L. monocytogenes to this type of disinfectant.Key points?Either SugE1 or SugE2 was sufficient for QACs resistance.?The functions of two sug genes overlap and compensate each other in QACs resistance.?SugR is the transcriptional suppressor of sugE1 and sugE2.?SugR regulates the sug locus by binding to the operon promoter. ? 2020, Springer-Verlag GmbH Germany, part of Springer Nature.