文献类型：期刊文献

英文题名：Colorimetric kinetic determination of potassium ions based on the use of a specific aptamer and catalytically active gold nanoparticles

作者：Li, Li[1];Li, Weiwei[1]

第一作者：李丽

通讯作者：Li, L[1]

机构：[1]Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China

第一机构：新乡学院化学化工学院

通讯机构：[1]corresponding author), Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China.|[110713]新乡学院化学化工学院;[11071]新乡学院;

年份：2015

卷号：182

期号：13-14

起止页码：2307-2312

外文期刊名：MICROCHIMICA ACTA

收录：;Scopus(收录号：2-s2.0-84941933893);WOS:【SCI-EXPANDED(收录号:WOS:000361394900026)】；

基金：All authors gratefully acknowledge the financial support of key scientific and technological project of Henan Province (122102210406).

语种：英文

外文关键词：Colorimetry; Aptamer; Potassiumion; Kinetic assay; SELEX

摘要：We describe a simple, highly sensitive, and selective colorimetric kinetic assay for the determination of potassium(I) by exploiting the specific recognition capability of an appropriate aptamer and catalytic signal amplification by gold nanoparticles (AuNPs). Amplification is based on the reduction of 4-nitrophenol by borohydride which is catalyzed by AuNPs. This leads to a color change of the solution from yellow to colorless, and the color change can be recognized with bare eyes or via photometry. The K(I)-selective aptamer is placed on the AuNPs and forms a tightly bound G-quadruplex with K(I) which partially masks the surface of the AuNPs and prevents 4-nitrophenol to be reduced at the catalytically active surface of the AuNPs. Hence, the rate of decoloration is retarded. The assay displays high selectivity for K(I) over other cations, has a linear response in the 0.1 nM to 10 mu M concentration range, and a detection limit as low as 0.06 nM. In addition, these findings pave the way to novel analytical methods based on the use of gold nanoparticle-catalyzed chemical reactions.