文献类型：期刊文献

英文题名：Characterization and horizontal transfer of qacH-associated class 1 integrons in Escherichia coli isolated from retail meats

作者：Jiang, Xiaobing[1];Xu, Yameng[1];Li, Yi[1];Zhang, Kun[1];Liu, Lei[1];Wang, Hailei[1];Tian, Jinhe[2];Ying, Hao[2];Shi, Lei[3];Yu, Tao[2]

第一作者：Jiang, Xiaobing

通讯作者：Yu, T[1]

机构：[1]Henan Normal Univ, Coll Life Sci, Xinxiang, Peoples R China;[2]Xinxiang Univ, Coll Life Sci & Technol, Xinxiang 453000, Peoples R China;[3]Jinan Univ, Inst Food Safety & Nutr, Guangzhou, Guangdong, Peoples R China

第一机构：Henan Normal Univ, Coll Life Sci, Xinxiang, Peoples R China

通讯机构：[1]corresponding author), Xinxiang Univ, Coll Life Sci & Technol, Xinxiang 453000, Peoples R China.|[1107115]新乡学院生命科学技术学院;[11071]新乡学院;

年份：2017

卷号：258

起止页码：12-17

外文期刊名：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85025101919);WOS:【SCI-EXPANDED(收录号:WOS:000410012500002)】；

基金：This work was supported by the National Natural Science Foundation of China (31601568), the Key Project of Natural Science of the Education Department of Henan Province, China (15A180006 and 16A180011) and the Key Scientific and Technological Project of Xinxiang (ZG15007). We are grateful to Minggui Wang, Huashan Hospital, Fudan University, for kindly providing the E. coli J53Az^r.

语种：英文

外文关键词：Escherichia coli; qac genes; Integrons; Horizontal transfer

摘要：The aim of this study was to examine the presence of genes responsible for resistance to quaternary ammonium compounds (QACs) and the association of qac genes with class 1 integrons in Escherichia coil isolated from retail meats. Among the 179 E. coil isolates tested, the minimum inhibitory concentrations (MICs) of benzalkonium chloride (BC) ranged from 4 to 64 g/mL. PCR assays indicated that QAC-resistance genes sugE(c), ydgE/ydgF, mdfA, emrE and qacE Delta 1 were commonly present (40.2%-88.3%) in these isolates, but qacE, qacF, qacH and sugE (p) were less prevalent (2.2%-28.5%). Seven different gene cassette arrangements were identified in 31 intI1-positive isolates. Three types of qacH-sul3-associated non-classic integrons were observed in four isolates: dfrAl2-orfF-aadA2-cm1A1-aadAl-qacH-15440-sul3, aadA2-cm1A1-aadAl-qacH-15440-sul3 and dfrAl-aadAl-qacH1S440-sul3. Non-classic class 1 integrons were located on plasmids of 100-150 kb in these four isolates. Our results demonstrated that the qacH-associated integrons located on 100 kb plasmids in two isolates could be transferred to an E. coil recipient, indicating the co-existence and co-dissemination of disinfectant and antimicrobial resistance genes among bacterial species.