文献类型：期刊文献

英文题名：Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus

作者：Yin, Mei[1];Hu, Dongfang[1];Li, Peng[2];Kong, Lingyun[3];Ning, Hongmei[1];Yue, Feng[2];Jiang, Jinqing[1];Wang, Xuannian[2]

第一作者：Yin, Mei

通讯作者：Wang, XN[1]

机构：[1]Henan Inst Sci & Technol, Coll Anim Sci & Technol, Xinxiang 453003, Henan, Peoples R China;[2]Xinxiang Univ, Coll Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China;[3]Xinxiangxian Forestry Bur, Xinxiangxian Agr & Anim Husb Bur, Xinxiang 453700, Henan, Peoples R China

第一机构：Henan Inst Sci & Technol, Coll Anim Sci & Technol, Xinxiang 453003, Henan, Peoples R China

通讯机构：[1]corresponding author), Xinxiang Univ, Coll Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China.|[1107115]新乡学院生命科学技术学院;[11071]新乡学院;

年份：2020

卷号：64

期号：1

起止页码：9-14

外文期刊名：JOURNAL OF VETERINARY RESEARCH

收录：;Scopus(收录号：2-s2.0-85091984211);WOS:【SCI-EXPANDED(收录号:WOS:000522624700002)】；

基金：The source of funding of research and the article were the Key Scientific Research Projects of the Universities in Henan Province (16A230001), the Programme for Science and Technology Innovation Talents in the Universities of Henan Province (14HASTIT026), and the Excellent Youth Foundation of the Henan Scientific Committee (174100510005).

语种：英文

外文关键词：classical swine fever virus; PK15; high-permissive cells; cell cloning; vaccine production

摘要：Introduction: Classical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the world. Attenuated live vaccines with CSFV antigens have played an important role in the prevention and control of the disease. Porcine kidney 15 (PK15) cells have been widely used for the propagation of CSFV, but this cell line is not efficient or homogeneously susceptible to viral infection. Material and Methods: To achieve a homogeneous PK15 cell line which enabled high titre replication of CSFV, we used the limiting dilution cell cloning method. Results: We developed two cell clones, PK15-1A6 and PK15-3B1, which respectively have high- and low-permissive phenotypes to CSFV infection. The PK15-1A6, PK15-3B1, and PK15 parent cells showed different characteristics in cell proliferation rate, susceptibility to CSFV infection, and CSFV production. The mean virus titres per millilitre reflected by TCID50 values in PK15-1A6, PK15-3B1, and PK15 parent cells were 10(6.85), 10(3.63), and 10(4.74), respectively. Conclusion: The PK15-1A6 cell clone is more permissive to CSFV infection than the PK15 parent cells. The screened high-permissive cells will be useful for CSFV propagation and vaccine development in vitro, and facilitate research on the pathogenicity of CSFV.