文献类型：期刊文献

英文题名：Genetic Variation of Wheat Landraces from China as Revealed by Gliadin and Microsatellite Markers

作者：Li, X. J.[1,2];Xu, X.[1,3];Li, X. Q.[1];Yang, X. M.[1];Liu, W. H.[1];Li, L. H.[1]

第一作者：Li, X. J.

通讯作者：Li, LH[1]

机构：[1]Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China;[2]Henan Inst Sci & Technol, Sch Life Sci & Technol, Xinxiang 453003, Peoples R China;[3]Xinxiang Univ, Dept Life Sci & Technol, Xinxiang 453003, Peoples R China

第一机构：Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

通讯机构：[1]corresponding author), Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China.

年份：2013

卷号：41

期号：4

起止页码：573-582

外文期刊名：CEREAL RESEARCH COMMUNICATIONS

收录：;Scopus(收录号：2-s2.0-84886937531);WOS:【SCI-EXPANDED(收录号:WOS:000325766400007)】；

基金：This study was supported by a grant from the 973 Project of China (No. 2011CB100104).

语种：英文

外文关键词：genetic diversity; gliadin; microsatellite; wheat landrace

摘要：A reborn interest has occurred during the last decade toward wheat landraces for broadening genetic basis of modern wheat cultivars. The investigation of molecular traits within and between existing landraces of wheat can help scientists to develop appropriate strategies for their efficient maintenance and exploitation. The present study dealt with the gliadin characterization of forty-seven wheat landraces collected from wheat mainly planted areas of China, each of which was represented by a sample of at least 43 individuals. Twelve accessions selected on the basis of gliadin analysis were investigated further using 21 SSR markers. The results proved that landraces of wheat are a mixture of variable individuals genetically distinguishable from each other. Twelve of the analyzed 47 accessions were observed to be homogeneous, while 35 (74.5%) of them were heterogeneous in their gliadin composition. In total, 122 gliadin pattern were observed. On average, 10.1% (Gst) of the total variation arose from differentiation among regions, and 89.9% was attributed to within-region variation. Furthermore, nineteen of the 21 SSR markers were polymorphic across all the populations. The total number of the amplified DNA products was 110, with a mean of 6.11 alleles per locus. The values of genetic diversity within each landrace population varied from 0.006 to 0.351. Implications for the management of this valuable genetic resource are discussed.