文献类型：期刊文献

英文题名：Sensitive label-free electrochemical immunoassay based on a redox matrix of gold nanoparticles/Azure I/multi-wall carbon nanotubes composite

作者：Sun, Ai-Li[1,2];Chen, Gai-Rong[2];Sheng, Qing-Lin[1];Zheng, Jian-Bin[1]

第一作者：Sun, Ai-Li

通讯作者：Zheng, JB[1]

机构：[1]NW Univ Xian, Shaanxi Prov Key Lab Electroanalyt Chem, Inst Analyt Sci, Xian 710069, Shaanxi, Peoples R China;[2]Xinxiang Univ, Dept Chem & Chem Engn, Xinxiang 453000, Peoples R China

第一机构：NW Univ Xian, Shaanxi Prov Key Lab Electroanalyt Chem, Inst Analyt Sci, Xian 710069, Shaanxi, Peoples R China

通讯机构：[1]corresponding author), NW Univ Xian, Shaanxi Prov Key Lab Electroanalyt Chem, Inst Analyt Sci, Xian 710069, Shaanxi, Peoples R China.

年份：2011

卷号：57

期号：1

起止页码：1-6

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20114114419512);Scopus(收录号：2-s2.0-80053606979);WOS:【SCI-EXPANDED(收录号:WOS:000297491100001)】；

基金：The authors appreciate the financial support from the National Natural Science Foundation of China (No. 20875076), the Natural Science Fundation of Henan Province (No.102300410185) and Science and Technology innovation Foundation of XinXiang University.

语种：英文

外文关键词：Label-free immunosensor; Multi-wall carbon nanotubes; Gold nanoparticles; Azure I; Carcinoembryonic antigen

摘要：A sensitive label-free electrochemical immunosensing platform was designed by a redox matrix of gold nanoparticles (GNPs), Azure land multi-wall carbon nanotubes (MWCNT) self-assemblying nanocomposite. To construct the immunosensor, MWCNT was first dispersed in Nafion (Nf) to obtain a homogeneous solution and then it was dropped on the surface of the gold electrode (Au). Then the positively-charged redox molecule, Azure I, was entrapped into MWCNT-Nf film to form a redox nanostructural membrane. Next, the negatively charged gold nanoparticles (GNPs) were assembled to the interface through the electrostatic force. Finally, carcinoembryonic antibody molecules could be absorbed into the GNPs/Azure I/MWCNT-Nf immobilization matrix. Using carcinoembryonic antigen (CEA) as a model protein, the electrochemical immunosensor exhibited good stability and reproducibility, as well as good selectivity and storage stability. This strategy presented a promising platform for sensitive and facile monitoring of CEA. (C) 2011 Elsevier B.V. All rights reserved.