文献类型：期刊文献

中文题名：SYBR Green I荧光检测痕量宿主DNA

英文题名：Detecting trace host DNA based on SYBR Green I fluorescence method

作者：蔺芳[1];付瑞敏[2];皮川真[3];时凯[3];叶刚[3];江景玉[3];陈慧珍[3]

第一作者：蔺芳

机构：[1]新乡学院生命科学与技术系;[2]河南教育学院人口与生命科学系;[3]邦和药业股份有限公司

第一机构：新乡学院生命科学技术学院

年份：2011

卷号：42

期号：7

起止页码：125-129

中文期刊名：东北农业大学学报

外文期刊名：Journal of Northeast Agricultural University

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：河南教育学院青年科研课题(20100103)

语种：中文

中文关键词：痕量DNA;SYBR;Green;I;荧光法;Southern印迹杂交

外文关键词：trace DNA; SYBR Green I; fluorescence method; Southern blotting

摘要：基于SYBR Green I荧光染料与双链DNA结合产生荧光的原理,建立一种痕量DNA荧光检测方法。采用不同稀释倍数的SYBR Green I荧光染料做DNA的标准曲线,确定SYBR Green I荧光染料的最佳稀释倍数。比较荧光法和Southern blotting杂交法定量DNA。结果表明,SYBR Green I荧光染料稀释倍数为1∶10 000时线性范围广,线性关系好(R2=0.9999);荧光法和Southern blotting杂交法可以相互印证。由此可知,SYBR Green I荧光法定量DNA是一种快速、稳定的痕量DNA检测方法。
A fluorescence method for trace amount DNA detection was established based on the principle that combination of SYBR Green I fluorescent dyes and double-stranded DNA will generate fluorescent.Using different dilution of SYBR Green I fluorescent dye to do DNA standard curve to confirm the optimization of dilution for the SYBR Green I fluorescence dye.Comparison of detection results of quantitative DNA analysis between fluorescence and Southern blotting hybridization.The results showed that it could obtain the wide linear range and better linear relationship（R2=0.9999） when the dilution of SYBR Green I fluorescent dye was 1∶10 000,and the methods of fluorescence hybridization and Southern blotting could permit each other.SYBR Green I fluorescent analysis of quantitative DNA is a fast and stable detection method of trace DNA.