文献类型：期刊文献

英文题名：Platinum nanozyme-encapsulated poly(amidoamine) dendrimer for voltammetric immunoassay of pro-gastrin-releasing peptide

作者：Zhi, Li-Juan[1];Sun, Ai-Li[1,2]

第一作者：职丽娟

通讯作者：Sun, AL[1]

机构：[1]Xinxiang Univ, Dept Chem & Chem Engn, Xinxiang 453000, Henan, Peoples R China;[2]Xinxiang Univ, Dept Pharm, Key Lab Nanocarbon Modified Film Technol Engn Hen, Xinxiang 453000, Henan, Peoples R China

第一机构：新乡学院化学化工学院

通讯机构：[1]corresponding author), Xinxiang Univ, Dept Chem & Chem Engn, Xinxiang 453000, Henan, Peoples R China.|[110713]新乡学院化学化工学院;[11071]新乡学院;

年份：2020

卷号：1134

起止页码：106-114

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20203609139081);Scopus(收录号：2-s2.0-85090149365);WOS:【SCI-EXPANDED(收录号:WOS:000579368600012)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Grant no: 51802275), the Key Scientific and Technological Research Project of Henan Province (Grant no.: 202102310298), and the Research Fund for Technological Innovative Scholar of Xinxiang University (China).

语种：英文

外文关键词：Electrochemical immunoassay; Pro-gastrin-releasing peptide; Platinum nanozyme; poly(amidoamine) dendrimer; Voltammetric measurement

摘要：An innovative electrochemical immunosensing platform was designed for the sensitive monitoring of lung cancer biomarker (pro-gastrin-releasing peptide; ProGRP) by using platinum nanoparticles encapsulated inside dendrimers (PtDEN) as enzymatic mimics for the signal amplification. PtDEN nanocomposites were prepared through a simple chemical reduction method with the assistance of NaBH4. Thereafter, PtDEN-labeled anti-ProGRP secondary antibody was launched for the detection of target analyte with a sandwich-type assay format on anti-ProGRP capture antibody-modified screen-printed carbon electrode. Accompanying formation of immunocomplex, the labeled PtDENs electrochemically oxidized 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide to produce a well-defined voltammetric signal within the applied potentials. Thanks to the high-efficient catalytic efficiency of platinum nanoparticles and high-loading ability of dendrimer, improved analytical features were acquired with PtDENs relative to platinum nanoparticles alone. Using PtDENs labeling strategy, the properties and factors influencing the analytical performance of electrochemical immunosensor were studied in detail. The strong bioconjugation of antibodies with the PtDENs caused a good repeatability and intermediate precision down to 7.64%. Under optimum conditions, the electrochemical immunosensor exhibited a dynamic linear range of 0.001-10 ng mL(-1) ProGRP with a detection limit of 0.86 pg mL(-1). Good selectivity and relatively long-term stability (>6 months) were achieved for target ProGRP. Significantly, the acceptable accuracy was gotten for analysis of ProGRP in human serum specimens referring to commercially available human ProGRP enzyme-linked immunosorbent assay (ELISA) method. (C) 2020 Elsevier B.V. All rights reserved.