文献类型：期刊文献

英文题名：Development of an immunochromatographic strip for the detection of antibodies against foot-and-mouth disease virus serotype O

作者：Yang, Suzhen[1,2];Yang, Jifei[1];Zhang, Gaiping[1];Wang, Xuannian[3];Qiao, Songlin[1,2];Zhao, Dong[1];Zhi, Yubao[1];Li, Xuewu[1];Xing, Guangxu[1];Luo, Jun[1];Fan, Jianming[1];Bao, Dengke[1,2]

第一作者：Yang, Suzhen

通讯作者：Zhang, GP[1]

机构：[1]Henan Acad Agr Sci, Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China;[2]Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Peoples R China;[3]Xinxiang Univ, Xinxiang 453003, Peoples R China

第一机构：Henan Acad Agr Sci, Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

通讯机构：[1]corresponding author), Henan Acad Agr Sci, Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China.

年份：2010

卷号：165

期号：2

起止页码：139-144

外文期刊名：JOURNAL OF VIROLOGICAL METHODS

收录：;Scopus(收录号：2-s2.0-77951025602);WOS:【SCI-EXPANDED(收录号:WOS:000277763700003)】；

基金：The study was supported by National Natural Science Foundation of China (no. 30730068) and the Chinese National High technology Research and Development Program (grant 2007AA100606). We also thank Dr. Norman A. Gregson for editorial assistance.

语种：英文

外文关键词：Foot-and-mouth disease; ELISA; Immunochromatographic strip

摘要：An immunochromatographic strip was developed for the serological detection of type 0 foot-and-mouth disease (FMD) in swine. In the strip, the expressed protein of VP1, the main protective antigen of FMDV, labeled with colloidal gold was used as the detector, the staphylococcal protein A (SPA) and swine anti-foot-and-mouth disease virus (FMDV) antibody were blotted on the nitrocellulose membrane for the test and control lines, respectively. 296 swine serum samples were collected to evaluate the characteristics of the strip in comparison with existing commercial liquid-phage blocking ELISA (LPB ELISA) kit and peptide ELISA kit. The strip was shown to be of high specificity and sensitivity. Furthermore, the dipstick assay based on the strip is rapid (5 min) and easy to perform with no requirement of professional skills, reagents nor equipment. This suggests that the immunochromatographic strip is an acceptable alternative for use in clinical laboratories lacking specialized equipment and for field diagnosis. (C) 2010 Elsevier B.V. All rights reserved.