文献类型：期刊文献

英文题名：Characterization and protective efficacy of a Salmonella pathogenicity island 2 (SPI2) mutant of Salmonella Paratyphi A

作者：Yin, Junlei[1];Cheng, Zhao[1];Wu, Yuchen[1];He, Qunli[2,3];Zhang, Jingjing[2];Yang, Zhanfeng[2];Ding, Shuming[2];Li, Jizong[4];Guo, Dongguang[1]

第一作者：殷俊磊

通讯作者：Yin, JL[1]

机构：[1]Xinxiang Univ, Sch Life Sci & Technol, 191 East Jinsui Rd, Xinxiang 453003, Henan, Peoples R China;[2]Zhengzhou Univ Ind Technol, Med Coll, Zhengzhou, Henan, Peoples R China;[3]Xinxiang Med Univ, Coll Basic Med, Xinxiang, Henan, Peoples R China;[4]Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Jiangsu, Peoples R China

第一机构：新乡学院生命科学技术学院

通讯机构：[1]corresponding author), Xinxiang Univ, Sch Life Sci & Technol, 191 East Jinsui Rd, Xinxiang 453003, Henan, Peoples R China.|[1107115]新乡学院生命科学技术学院;[11071]新乡学院;

年份：2019

卷号：137

外文期刊名：MICROBIAL PATHOGENESIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000502891200057)】；

基金：Henan of Programs for Science and Technology Development (Grant 192102310416, 192102110066); PhD Programs Foundation of Xinxiang University (Grant 1366020121); and Key Scientific Research Projects for Higher Education of Henan Province (19A230009).

语种：英文

外文关键词：Salmonella paratyphi A; Paratyphoid fever; Salmonella pathogenicity island 2; Virulence; Live attenuated vaccine

摘要：Paratyphoid fever caused by Salmonella Paratyphi A is a serious public health problem in many countries. In order to and develop a live attenuated candidate vaccine of Salmonella Paratyphi A, a Salmonella pathogenicity island 2 (SPI2, approximate 40 kb) deletion mutant of Salmonella Paratyphi A was constructed by lambda Red recombination, then the biological characteristics and protective ability of the Salmonella Paratyphi A SPI2 mutant were evaluated. Our results showed that the growth and biochemical properties of the SPI2 mutant were consistent with that of its parent strain, and the mutant was stable with the loss of SPI2. The mice lethal test showed that the virulence of the SPI2 mutant was significantly decreased, it can colonize and persistent more than 14 days in the liver and spleen of mice. Vaccination with the SPI2 mutant in mice revealed no significant effect on body weight and clinical symptoms compared to control animals, and specific humoral and cellular immune responses were also significantly induced. Immunization of mice offered efficient protection against Salmonella Paratyphi A strain challenge at 14 days post vaccination based on mortality and clinical symptoms relative to control group. Overall, these findings suggested that SPI2 plays an important role in pathogenicity of Salmonella Paratyphi A, and the SPI2 mutant showed its potential to develop a live attenuated vaccine candidate.