详细信息
口蹄疫抗原决定簇融合基因转化生菜及表达
Transformation and Expression of FMD Antigenic-determinant Fused Gene in Lactuca sativa var. capatata
文献类型:期刊文献
中文题名:口蹄疫抗原决定簇融合基因转化生菜及表达
英文题名:Transformation and Expression of FMD Antigenic-determinant Fused Gene in Lactuca sativa var. capatata
作者:邓小莉[1];常景玲[2]
第一作者:邓小莉
机构:[1]平原大学应用生物系分子生物学实验室;[2]河南科技学院生物工程系分子生物学重点实验室
第一机构:新乡学院生命科学技术学院
年份:2007
卷号:24
期号:5
起止页码:609-613
中文期刊名:植物学通报
外文期刊名:Chinese Bulletin of Botany
收录:北大核心:【北大核心2004】;CSCD:【CSCD2011_2012】;
基金:福建省科技计划项目(No.JY-03-B-30-20)
语种:中文
中文关键词:根癌农杆菌;口蹄疫抗原决定簇;基因表达;生菜
外文关键词:Agrobacterium tumefaciens, FMDV antigenic determinant, gene expression, Lactuca sativa var. capatata
摘要:以3-5天苗龄的散叶大速生生菜(Lactucasativavar.capatata)无菌苗子叶为外植体,通过根癌农杆菌介导,将携带O型和A型口蹄疫抗原决定簇融合基因O21-O14-A21-HBcAg导入生菜。研究结果表明,含有20mg.L-1潮霉素(Hyg)的S2培养基(MS+1.5mg.L-16-BA+0.2mg.L-1IAA+20mg.L-1Hyg+300mg.L-1Cb)为子叶外植体转化后诱导愈伤和芽再生的最适培养基,经抗性筛选,将抗性芽切下于S3培养基(1/2MS+20mg.L-1Hyg)上诱导生根。通过PCR和Southern杂交分析证明,基因已经整合到生菜基因组中。RT-PCR检测初步表明,O21-O14-A21-HBcAg基因可以在生菜中正常转录。
The FMD antigenic-determinant fused gene (021-014 -A21-HbcAg) was introduced into lettuce (Lactuca sativa var. capatata) by co-culturing excised cotyledon explants with Agrobacterium tumefaciens. The best transformation efficiency was obtained on co-culture with the selective medium S2 (MS + 1.5 mg·L^-1 6-BA + 0.2 mg·L^-1 IAA + 500 mg·L^-1 Cb) containing 20 mg·L^-1hygromycin. Roots were induced on S3 (1/2 MS + 20 mg·L^-1Hyg). Integration of FMD antigenic-determinant fused gene O21-O14 -A21-HBcAg into the genome of lettuce plants was confirmed by PCR and Southern blotting analysis. Expression of the fused gene at the transcriptional level was shown by RT-PCR.
参考文献:
正在载入数据...