文献类型：期刊文献

英文题名：Homogeneous capture and heterogeneous separation of proteins by PEG-functionalized ionic liquid-water systems

作者：Yao, Wenhui[1,2];Wang, Huiyong[1];Pei, Yuanchao[1];Chen, Yuehua[1];Li, Zhiyong[1];Wang, Jianji[1]

第一作者：姚文惠;Yao, Wenhui

通讯作者：Wang, JJ[1]

机构：[1]Henan Normal Univ, Minist Educ, Key Lab Green Chem Media & React,Sch Chem & Chem, Collaborat Innovat Ctr Henan Prov Green Mfg Fine, Xinxiang 453007, Henan, Peoples R China;[2]Xinxiang Univ, Sch Civil Engn, Xinxiang 453003, Henan, Peoples R China

第一机构：Henan Normal Univ, Minist Educ, Key Lab Green Chem Media & React,Sch Chem & Chem, Collaborat Innovat Ctr Henan Prov Green Mfg Fine, Xinxiang 453007, Henan, Peoples R China

通讯机构：[1]corresponding author), Henan Normal Univ, Minist Educ, Key Lab Green Chem Media & React,Sch Chem & Chem, Collaborat Innovat Ctr Henan Prov Green Mfg Fine, Xinxiang 453007, Henan, Peoples R China.

年份：2017

卷号：7

期号：19

起止页码：11297-11303

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20170803378818);Scopus(收录号：2-s2.0-85013344057);WOS:【SCI-EXPANDED(收录号:WOS:000395867900011)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 21573060 and 21673068), the Program for Innovative Research Team in Science and Technology in University of Henan Province (No. 16IRTSTHN002), and the Plan for Scientific Innovation Talent of Henan Province (No. 144200510004).

语种：英文

外文关键词：Circular dichroism spectroscopy - Dichroism - Efficiency - Ionic liquids - Mammals - pH - Polyethylene glycols - Solvent extraction

摘要：Extraction and separation of proteins is important for their interesting applications as continuous enzymatic reaction and/or product separation systems. In this work, a new highly efficient homogeneous capture and heterogeneous liquid-liquid separation strategy is presented for the extraction of proteins from aqueous solution by PEG (poly(ethylene glycol))-functionalized ionic liquids, which have a lower critical solution temperature (LCST) phase behaviour in water. The factors influencing the solvent extraction process such as homogeneous capture time, pH value of aqueous phase and water content in the ionic liquids were investigated systematically. It was found that homogeneous capture of the proteins was quite fast, typically 1 min was enough to achieve equilibrium, and the extraction efficiency was greatly affected by water phase pH value and water content in the ionic liquid phases. Under optimal conditions, the single-step extraction efficiency by [PEG(800)(mim) (2)][NTf2] (2) was higher than 95% for most of the studied proteins (cytochrome c, myoglobin, hemoglobin, lysozyme and papain), but it was only 8% for bovine serum and 2% for peroxidase. Based on the significant differences in the extraction efficiencies observed, selective separation of the proteins from bovine serum or/and peroxidase was successfully performed using the IL/water mixture. In addition, circular dichroism and FT-IR spectroscopy were used to probe the structure and conformation change of the proteins, and the extraction mechanism was also discussed from the point of view of the electrostatic and hydrophobic/hydrophilic interactions of proteins with ionic liquids.