文献类型：期刊文献

中文题名：大豆异戊烯焦磷酸异构酶基因的电子克隆及生物信息学分析

英文题名：Electronic Cloning and Bioinformatical Analysis of IPI Gene from Glycine max

作者：魏琦超[1];畅丽萍[2];薛晓锋[1];周岩[1]

第一作者：魏琦超

机构：[1]河南科技学院生命科技学院;[2]新乡学院建筑工程系

第一机构：河南科技学院生命科技学院,新乡453003

年份：2010

期号：11

起止页码：96-100

中文期刊名：生物技术通报

外文期刊名：Biotechnology Bulletin

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：教育部回国留学人员科研启动基金项目(教外司留[2008]890);河南省科技厅基础与前沿技术研究计划项目(072300430120)

语种：中文

中文关键词：大豆;异戊烯焦磷酸异构酶;电子克隆;生物信息学分析

外文关键词：Glycine max Isopentenyl diphosphate isomerase（IPI）Electronic clone Bioinformatics analysis

摘要：利用电子克隆方法获得大豆异戊烯焦磷酸异构酶基因(IPI)的cDNA序列,并采用生物信息学方法对该基因及其编码蛋白的一般理化性质、疏水性、信号肽、二级结构和亚细胞定位等方面进行了预测和分析。结果表明,大豆异戊烯焦磷酸异构酶基因的cDNA序列全长1384bp,包含一个906bp的ORF,编码301个氨基酸。大豆IPI酶为一亲水性的非分泌蛋白,α-螺旋和无规则卷曲是其主要的二级结构,该酶定位于叶绿体。
An cDNA sequence of isopentenyl diphosphate isomerase（IPI）gene from Glycine max was cloned by electronic cloning.Some characters of the IPI and encoded protein sequence were predicted and analyzed by the methods of bioinformatics in the following aspects,including the general physical and chemical properties,hydrophobicity,signal peptide,secondary structure and localization sites in cells.Results showed that the full-length of IPI from Glycine max was 1 384 bp long and contained a complete ORF（906 bp）which encoded 301 amino acid.The isopentenyl diphosphate isomerase（IPI）of Glycine max was a dydrophilic and Non-secretory protein,which located in chloroplast.The main secondary structure elements of IPI were alpha helix and random coil.