文献类型：期刊文献

英文题名：Integrated microRNA and mRNA sequencing analysis of age-related changes to mouse thymic epithelial cells

作者：Jia, Hong-Ling[1];Zeng, Xiao-Qin[2];Huang, Feng[2];Liu, Ya-Meng[1];Gong, Bi-Shuang[1];Zhang, Kai-Zhao[1];Zeng, Jiang-Hui[3];Guo, Dong-Guang[4];Wang, Zhuo-Ya[5];Li, Yu-Gu[1]

第一作者：Jia, Hong-Ling

通讯作者：Li, YG[1];Wang, ZY[2]

机构：[1]South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China;[2]Guangzhou Women & Childrens Med Ctr, Guangzhou, Guangdong, Peoples R China;[3]Guangxi Med Univ, Dept Clin Lab, Affiliated Hosp 3, Nanning, Guangxi Zhuang, Peoples R China;[4]Xinxiang Univ, Sch Life Sci & Technol, Biotechnol Res Ctr, Xinxiang, Henan, Peoples R China;[5]Guangdong Pharmaceut Univ, Sch Basic Courses, Guangdong Prov Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Guangdong, Peoples R China

第一机构：South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

通讯机构：[1]corresponding author), South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China;[2]corresponding author), Guangdong Pharmaceut Univ, Sch Basic Courses, Guangdong Prov Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Guangdong, Peoples R China.

年份：2018

卷号：70

期号：7

起止页码：678-690

外文期刊名：IUBMB LIFE

收录：;Scopus(收录号：2-s2.0-85046378608);WOS:【SCI-EXPANDED(收录号:WOS:000438343900011)】；

基金：This work was supported by National Natural Science Foundation of China (No. 31572475).

语种：英文

外文关键词：mouse; thymic epithelial cells; miRNA sequencing analysis; mRNA sequencing analysis; thymic involution

摘要：MicroRNAs (miRNAs) play key roles in the regulation of gene expression during multiple physiological processes, including early development, differentiation, and ageing. However, their involvement in age-related thymic involution is not clear. In this study, we profiled the global transcriptome and miRNAome of thymic epithelial cells in 1- and 3-month-old male and female mice, and predicted the possible transcription factors and target genes of the four most significantly differentially expressed miRNAs (DEMs) (miR-183-5p, miR-199b-5p, miR-205-5p, and miR-200b-3p) by performing bioinformatics analyses. We also evaluated the relationships between the significantly DEMs and mRNAs. We performed quantitative polymerase chain reaction to confirm the changes in the expression of the miRNAs and their predicted target genes. We found that miR-183-5p, miR-199b-5p, miR-205-5p, and miR-200b-3p can be used as a biomarker group for mouse thymus development and involution. In addition, the predicted target genes (Ptpn4, Slc2a9, Pkib, Pecam1, and Prkdc), which were identified by mRNA sequencing analysis, were mainly involved in growth, development, and accelerated senescence. In conclusion, miRNAs and their predicted target genes likely play important roles in thymus development and involution. To the best of our knowledge, this is the first study to systematically analyze the relevance of miRNAs and their targets by mRNA sequencing in mouse thymic epithelial cells. (c) 2018 IUBMB Life, 70(7):678-690, 2018