文献类型：期刊文献

英文题名：Colorimetric detection of DNA at the nanomolar level based on enzyme-induced gold nanoparticle de-aggregation

作者：Liu, Qingling[1];Li, Li[1];Zhao, Yan[2];Chen, Zhengbo[2]

第一作者：刘清玲

通讯作者：Li, L[1];Chen, ZB[2]

机构：[1]Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China;[2]Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China

第一机构：新乡学院化学化工学院

通讯机构：[1]corresponding author), Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China;[2]corresponding author), Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China.|[110713]新乡学院化学化工学院;[11071]新乡学院;

年份：2018

卷号：185

期号：6

外文期刊名：MICROCHIMICA ACTA

收录：;Scopus(收录号：2-s2.0-85046894897);WOS:【SCI-EXPANDED(收录号:WOS:000434655900013)】；

基金：All authors gratefully acknowledge the financial support of Scientific Research Project of Beijing Educational Committee (Grant No. KM201710028009).

语种：英文

外文关键词：Exonuclease III; Nucleic acid detection; Colorimetric assay; DNA recycling; Amplification; Absorbance; Serum samples

摘要：The authors describe a colorimetric method for the determination of DNA based on the deaggregation of gold nanoparticles (AuNPs) induced by exonuclease III (Exo III). DNA amplification is accomplished by Exo III to generate large quantities of the residual DNA. Residual DNA tethers onto the surfaces of AuNPs which prevents their aggregation. Hence, the color of the solution is red. However, in the absence of DNA, salt-induced aggregation is not prevented, and the bluish-purple color of the aggregated AuNPs is observed. The ratio of absorbances at 525 and 625 nm increases up to 150 nM DNA concentrations, and the LOD is as low as 3.0 nM. It is shown that the presence of 300 nM concentrations of random DNA (with a mass up to 10-fold that of target DNA) does not interfere. The method was successfully applied to the analysis of DNA in spiked serum samples. The method is simple, reliable, and does not require complicated amplification steps and expensive instrumentation.