文献类型：期刊文献

英文题名：A label-free electrochemical immunosensor based on AuNPs/GO-PEI-Ag-Nf for olaquindox detection in feedstuffs

作者：Liu, Dan[1];Wang, Aiping[1,4];Zhou, Jingming[1];Wang, Xuannian[2];Liu, Hongliang[1];Ding, Peiyang[1];Zhang, Ying[1];Zhu, Xifang[1];Zhou, Yiting[1];Zhang, Gaiping[1,3]

第一作者：Liu, Dan

通讯作者：Zhang, GP[1];Zhang, GP[2]

机构：[1]Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China;[2]Xinxiang Univ, Sch Life Sci & Basic Med, Xinxiang 453003, Henan, Peoples R China;[3]Peking Univ, Sch Adv Agr Sci, Beijing, Peoples R China;[4]Key Lab Immunobiol Henan Prov, Zhengzhou, Henan, Peoples R China

第一机构：Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China

通讯机构：[1]corresponding author), Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China;[2]corresponding author), Peking Univ, Sch Adv Agr Sci, Beijing, Peoples R China.

年份：2022

卷号：177

外文期刊名：MICROCHEMICAL JOURNAL

收录：;Scopus(收录号：2-s2.0-85126065488);WOS:【SCI-EXPANDED(收录号:WOS:000793743600007)】；

基金：Acknowledgements This research was supported by the National Key Research and Development Plan of China (2018YFC1602902) and the National Key Research and Development Program of China (2019YFC1604501) .

语种：英文

外文关键词：Polyethyleneimine decorated silver-graphene; oxide nanocomposites; Gold nanoparticles; Electrochemical immunosensor; Olaquindox

摘要：For the first time, a label-free electrochemical immunosensor was developed to detect olaquindox (OLA) in feedstuffs using graphene oxide (GO) nanocomposites (GO-PEI-Ag) decorated by silver nanoparticles (AgNPs) and functionalized by polyethyleneimine (PEI) as electrode modification materials. In this work, GO with huge specific surface area was used as a carrier to load abundant AgNPs, which exhibited good redox activity and perfect conductivity. The stability of GO-Ag in solution was ameliorated by PEI, which was utilized as a solubilizer. Nafion (Nf) was applied to attach tightly and uniformly GO-PEI-Ag nanomaterials on the electrode surface. The gold nanoparticles (AuNPs) were electrodeposited on the GO-PEI-Ag-Nf modified electrode surface for signal amplification and antibody immobilization. Above all, the electrochemical signal significantly improved. Under the optimal conditions, the signal intensity showed linear correlation with the logarithm of OLA concentration ranging from 0.01 ng mL-1 to 150 ng mL(-1), along with the low detection limit of 4.8 pg mL(-1). Moreover, the analytical performance in feedstuff samples was evaluated, showing favorable recovery of 90.0%100.2% with RSD of 2.2%-6.9%. The fabricated biosensor can provide a suitable approach to measure OLA and is promising to detect other additives in feedstuffs.