详细信息
Proteomic analysis of the antimicrobial effects of sublethal concentrations of thymol on Salmonella enterica serovar Typhimurium ( EI收录)
文献类型:期刊文献
英文题名:Proteomic analysis of the antimicrobial effects of sublethal concentrations of thymol on Salmonella enterica serovar Typhimurium
作者:Qi, Yonghua[1,2,3]; Zhao, Wei[2]; Wang, Tao[1]; Pei, Fangying[1]; Yue, Min[2]; Li, Feng[2]; Liu, Xingyou[4]; Wang, Xuannian[4]; Li, Hongquan[1]
第一作者:Qi, Yonghua
通讯作者:Li, Hongquan
机构:[1] College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China; [2] College of Medicine, Xinxiang University, Xinxiang, Henan, 453003, China; [3] College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, China; [4] College of Life Science and Technology, Xinxiang University, Xinxiang, Henan, 453003, China
第一机构:College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China
年份:2020
卷号:104
期号:8
起止页码:3493-3505
外文期刊名:Applied Microbiology and Biotechnology
收录:EI(收录号:20200908214080);Scopus(收录号:2-s2.0-85079882378)
语种:英文
外文关键词:High resolution transmission electron microscopy - Antibiotics - Salmonella - Molecular biology - Essential oils - Phenols
摘要:Salmonella enterica serovar Typhimurium is an important foodborne pathogen that causes serious and extensive food contamination as well as disease and death worldwide. Considering the increasing severity of antibiotic resistance, antibiotic alternatives are urgently needed. As a natural biocide and a component of some essential oils from herbs, thymol is capable of killing various bacteria through a potentially unique mechanism, although the targets of thymol have not been completely elucidated. In this study, the variation in the whole proteome of Salmonella after thymol stress was evaluated using the SWATH multiplex technique. The strain Salmonella Typhimurium CVCC541 was treated with a sublethal concentration (75?μg/mL) of thymol, which rapidly increased the permeability of bacterial membranes at the tested concentration. Thymol destroyed the integrity of the bacterial membrane, as observed by transmission electron microscopy. The proteomes of the treated and untreated cells were characterized after an 8-h treatment. The proteomic analysis of thymol-treated cells indicated that 144 proteins exhibited upregulation or downregulation compared with the control cells, particularly those involved in cellular structure and metabolism. The results of this study showed that thymol may play an antimicrobial role in altering the membrane permeability, virulence change, and antioxidant response of Salmonella Typhimurium. The results of the present study provide an improved understanding of the proteomic response of Salmonella Typhimurium to thymol stress, including the identification of promising targets for the future exploration of innovative approaches to control Salmonella Typhimurium. ? 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
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