文献类型：期刊文献

英文题名：Proteomic analysis of the antimicrobial effects of sublethal concentrations of thymol on Salmonella enterica serovar Typhimurium

作者：Qi, Yonghua[1,2,3];Zhao, Wei[2];Wang, Tao[1];Pei, Fangying[1];Yue, Min[2];Li, Feng[2];Liu, Xingyou[4];Wang, Xuannian[4];Li, Hongquan[1]

第一作者：Qi, Yonghua

通讯作者：Li, HQ[1]

机构：[1]Shanxi Agr Univ, Coll Anim Sci & Vet Med, Taigu 030801, Shanxi, Peoples R China;[2]Xinxiang Univ, Coll Med, Xinxiang 453003, Henan, Peoples R China;[3]Henan Inst Sci & Technol, Coll Anim Sci, Xinxiang 453003, Henan, Peoples R China;[4]Xinxiang Univ, Coll Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China

第一机构：Shanxi Agr Univ, Coll Anim Sci & Vet Med, Taigu 030801, Shanxi, Peoples R China

通讯机构：[1]corresponding author), Shanxi Agr Univ, Coll Anim Sci & Vet Med, Taigu 030801, Shanxi, Peoples R China.

年份：2020

卷号：104

期号：8

起止页码：3493-3505

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000518132100001)】；

基金：This study was financed by the Postdoctoral Research Funding of Shanxi Province, the Postdoctoral Research Funding of Shanxi Agricultural University, the Natural Science Foundation of Henan Province (162300410109), the Key Research Project of Henan Provincial Department of Education (19A230008), and the Research Program of Xinxiang University (XXUTD201701106 and 1333020286).

语种：英文

外文关键词：Salmonella typhimurium; Thymol; Quantitative proteomics; SWATH; Virulence factor

摘要：Salmonella enterica serovar Typhimurium is an important foodborne pathogen that causes serious and extensive food contamination as well as disease and death worldwide. Considering the increasing severity of antibiotic resistance, antibiotic alternatives are urgently needed. As a natural biocide and a component of some essential oils from herbs, thymol is capable of killing various bacteria through a potentially unique mechanism, although the targets of thymol have not been completely elucidated. In this study, the variation in the whole proteome of Salmonella after thymol stress was evaluated using the SWATH multiplex technique. The strain Salmonella Typhimurium CVCC541 was treated with a sublethal concentration (75 mu g/mL) of thymol, which rapidly increased the permeability of bacterial membranes at the tested concentration. Thymol destroyed the integrity of the bacterial membrane, as observed by transmission electron microscopy. The proteomes of the treated and untreated cells were characterized after an 8-h treatment. The proteomic analysis of thymol-treated cells indicated that 144 proteins exhibited upregulation or downregulation compared with the control cells, particularly those involved in cellular structure and metabolism. The results of this study showed that thymol may play an antimicrobial role in altering the membrane permeability, virulence change, and antioxidant response of Salmonella Typhimurium. The results of the present study provide an improved understanding of the proteomic response of Salmonella Typhimurium to thymol stress, including the identification of promising targets for the future exploration of innovative approaches to control Salmonella Typhimurium.