文献类型：期刊文献

英文题名：Porous platinum nanotubes labeled with hemin/G-quadruplex based electrochemical aptasensor for sensitive thrombin analysis via the cascade signal amplification

作者：Sun, Aili[1];Qi, Qingan[2,3,4];Wang, Xuannian[1];Bie, Ping[2,3]

通讯作者：Sun, AL[1]

机构：[1]Xinxiang Univ, Dept Chem & Chem Engn, Xinxiang 453000, Peoples R China;[2]Third Mil Med Univ, Southwest Hosp, Chongqing 400038, Peoples R China;[3]Third Mil Med Univ, Inst Hepatobilitary Surg, Chongqing 400038, Peoples R China;[4]371th Hosp PLA, Dept Gen Surg, Xinxiang 453000, Peoples R China

第一机构：新乡学院化学化工学院

通讯机构：[1]corresponding author), Xinxiang Univ, Dept Chem & Chem Engn, Xinxiang 453000, Peoples R China.|[110713]新乡学院化学化工学院;[11071]新乡学院;

年份：2014

卷号：57

起止页码：16-21

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20140917380759);Scopus(收录号：2-s2.0-84896714158);WOS:【SCI-EXPANDED(收录号:WOS:000334086100003)】；

基金：The authors would like to thank the National Natural Science Foundation of China (No. 31272539), the Natural Science Foundation of Henan Province (No. 102300410185), the Ph.D. Foundation Project of XinXiang University.

语种：英文

外文关键词：Porous PtNTs; Hemin/G-quadruplex; Glucose dehydrogenase; Cascade signal amplification; Electrochemical aptasensor

摘要：For the first time, a sensitive electrochemical aptasensor for thrombin (TB) was developed by using porous platinum nanotubes (PtNTs) labeled with hemin/G-quadruplex and glucose dehydrogenase (GDH) as labels. Porous PtNTs with large surface area exhibited the peroxidase-like activity. Coupling with GDH and hemin/G-quadruplex as NADH oxidase and HRP-mimicking DNAzyme, the cascade signal amplification was achieved by the following ways: in the presence of glucose and NAD(+) in the working buffer, GDH electrocatalyzed the oxidation of glucose with the production of NADH. Then, hemin/ G-quadruplex as NADH oxidase catalyzed the oxidation of NADH to in situ generate H2O2. Based on the corporate electrocatalysis of PtNTs and hemin/G-quadruplex toward H2O2, the electrochemical signal was significantly amplified, allowing the detection limit of TB down to 0.15 pM level. Moreover, the proposed strategy was simple because the intercalated hemin offered the readout signal, avoiding the adding of additional redox mediator as signal donator. Such an electrochemical aptasensor is highly promising for sensitive detection of other proteins in clinical diagnostics. (C) 2014 Elsevier B.V. All rights reserved.