文献类型：期刊文献

英文题名：Colorimetric detection of glutathione based on its inhibitory effect on the peroxidase-mimicking properties of WS2 nanosheets

作者：Li, Li[1];Wang, Qianna[2];Chen, Zhengbo[2]

第一作者：李丽

通讯作者：Chen, ZB[1]

机构：[1]Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China;[2]Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China

第一机构：新乡学院化学化工学院

通讯机构：[1]corresponding author), Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China.

年份：2019

卷号：186

期号：4

外文期刊名：MICROCHIMICA ACTA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000462640200001)】；

基金：All authors gratefully acknowledge the financial support of Science and Technology Innovation Funds of Xinxiang University (Grant No. 15ZP05), and Science Technology Open Cooperation Program of Henan Province of China (Grant No. 182106000029).

语种：英文

外文关键词：Glutathione detection; Colorimetric assay; Tungsten disulfide; 3,3 ',5,5 '-tetramethylbenzidine-H2O2 system; High sensitivity; Visual detection; Serum samples

摘要：A highly sensitive colorimetric assay is described for the determination of glutathione (GSH). It is based on the use of tungsten disulfide (WS2) which is a peroxidase mimic. It catalyzes the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) by hydrogen peroxide to form a blue-colored product (oxTMB) at near neutral pH values. In the presence of the analyte GSH, it is transformed to its oxidized form (GSSG), while the blue oxTMB is increasingly reduced and eventually is converted to colorless TMB. This can readily be detected with bare eyes. GSH can be determined spectroscopically (at 652nm) by this method at concentrations down to 61 pM, and the response is linear in the 100 pM to 10nM GSH concentration range. The assay is cost-effective and simple. Conceivably, it provides a promising tool for the determination of GSH in food and medical samples.