文献类型：期刊文献

英文题名：Apoptotic investigation of brain tissue cells in dogs naturally infected by canine distemper virus

作者：Pan, Yaoqian[1];Wang, Shuai[2];Li, Peng[1];Yue, Feng[1];Zhang, Yanfang[1];Pan, Bo[3];Liu, Xingyou[1]

第一作者：Pan, Yaoqian

通讯作者：Liu, XY[1];Pan, B[2]

机构：[1]Xinxiang Univ, Sch Life Sci & Basic Med, Xinxiang 453003, Henan, Peoples R China;[2]Xinxiang Univ, Sch Basic Med Sci, Xinxiang, Henan, Peoples R China;[3]Univ South Dakota, Sanford Sch Med, Div Basic Biomed Sci, Vermillion, SD 57069 USA

第一机构：新乡学院

通讯机构：[1]corresponding author), Xinxiang Univ, Sch Life Sci & Basic Med, Xinxiang 453003, Henan, Peoples R China;[2]corresponding author), Univ South Dakota, Sanford Sch Med, Div Basic Biomed Sci, Vermillion, SD 57069 USA.|[11071]新乡学院;

年份：2021

卷号：18

期号：1

外文期刊名：VIROLOGY JOURNAL

收录：;Scopus(收录号：2-s2.0-85112373364);WOS:【SCI-EXPANDED(收录号:WOS:000685305900001)】；

基金：This project was supported by awards No. 202102110094 from the Science and Technology Project of Henan Province.

语种：英文

外文关键词：Apoptosis; Canine distemper virus; Astrocyte; Oligodendrocyte; Demyelination

摘要：Background Canine distemper caused by canine distemper virus that belongs to the Morbillivirus genus of the Paramyxoviridae family is still a global epidemic significant infectious disease, especially in pet dogs in China and serious harm to the development of the dog industry. It has been known that apoptosis caused by the canine distemper virus can show in culture cells, lymphoid tissues, and the cerebellum. However, its occurrence in brain tissue cells remains unclear. To investigate the relationship among canine distemper infecting brain tissues, apoptosis in brain tissue cells, and demyelinating pathogenesis was investigated. Methods 16 naturally infected dogs that exhibited clinical signs of CD and tested positive for the anti-CDV monoclonal antibody and six healthy dogs that served as the control, were used in the research. Brain specimens were divided into the cerebrum, brain stem, and cerebellum embedded in paraffin and made the sections respectively. Approximately 5 mu m-thick sections were stained by hematoxylin-eosin, methyl green pyronin, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling technique, and immunohistochemistry. CDV nucleocapsid protein was detected by immune streptavidin-biotinylated peroxidase complex. Results Alterations in the brain tissues of CDV-infected dogs involved both various cells and nerve fibers. CDV had varying degrees of cytotropism to all brain tissue cells; apoptosis also occurred in all brain cells, especially in the endothelia of cerebral vessels, astrocytes, oligodendrocytes, and ependymal cells, the more serious infection, the more obvious apoptosis. Serious infections also involved the pyramidal and Purkinje cells. The nervous fibers exhibited demyelinating lesions (showed small multifocal vacuole), and some axonal neuron atrophy gradually disappeared (formed large vacuole). Conclusions Apoptosis in brain tissue cells was mainly related to the propagation path and cytotropism of CDV. The apoptosis of astrocytes, oligodendrocytes, and some neurons may play a significant role in the demyelinating pathogenesis in dogs with acute canine distemper. A lot of diverse nervous signs shown in the clinic may be related to different neuron apoptosis.