文献类型：期刊文献

中文题名：鲍曼不动杆菌质粒介导喹诺酮耐药机制研究

英文题名：Detection of plasmid-mediated quinolone resistance genes in Acinetobacter baumannii isolates

作者：姜晓冰[1];徐雅梦[1];于涛[2];王海磊[1];石磊[3]

第一作者：姜晓冰

机构：[1]河南师范大学生命科学学院;[2]新乡学院生命科学技术学院;[3]华南理工大学轻工与食品学院

第一机构：河南师范大学生命科学学院,新乡453007

年份：2016

卷号：41

期号：5

起止页码：382-387

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：河南省高等学校重点科研项目(No.15A180006);河南师范大学博士科研启动费支持课题(No.01046500152);河南师范大学青年科学基金资助(No.5101049279083)

语种：中文

中文关键词：鲍曼不动杆菌;质粒介导喹诺酮耐药;超广谱β-内酰胺酶

外文关键词：Acinetobacter baumannii; Plasmid-mediated quinolone resistance; Extended spectrum β-lactamases

摘要：目的调查临床分离的鲍曼不动杆菌中质粒介导喹诺酮耐药(PMQR)基因及超广谱β-内酰胺酶(ESBLs)基因的分布情况,并对PMQR阳性菌株中染色体介导的喹诺酮耐药机制进行分析。方法采用琼脂二倍稀释法测定菌株对环丙沙星和左氧氟沙星的药物敏感性;PCR检测qnr A、qnr B、qnr C、qnr D、qnr S、aac(6’)-Ib-cr和qep A,对PMQR阳性菌株扩增blaTEM、blaSHV、blaCTX-M和blaPER,同时扩增测序分析染色体基因gyr A、gyr B、par C和par E的突变情况;接合转移试验验证PMQR与bla基因的转移性。结果 91株鲍曼不动杆菌中有2株携带qnr B4基因。2株PMQR阳性菌株均接合转移成功,qnr B4和blaCTX-M-14、blaSHV-12基因可以通过质粒共同转移。PMQR阳性菌株均在Gyr A亚基和Par C亚基存在氨基酸突变,其相应的接合子以及受体菌均未发现突变。结论临床分离的鲍曼不动杆菌中存在qnr B基因,qnr与bla基因能共同转移,造成多重耐药的传播。
Objective To investigate the prevalence of plasmid-mediated quinolone resistance （PMQR） among clinical Acinetobacter baumannii, and the presence of extended spectrum β-lactamase （ESBL） genes and mutations in quinolone resistance-determining regions （QRDRs） in PMQR-positive isolates. Methods Antimicrobial susceptibility test was performed using the agar dilution method. Isolates were detected the presence of qnrA, qnrB, qnrC, qnrD, qnrS, aac（6′）-Ib-cr, qepA, blaTEM, blaSHV, blaCTX-M, and blaPER. Mutations in gyrA, gyrB, parC, and parE genes were identified by DNA sequencing of their PCR products. The transfer of PMQR and bla genes was studied by performing conjugation experiments. Results Among the 91 isolates of A. baumannii, only two isolates carried qnrB4 gene. Quinolone resistance could be transferred from both of the PMQR-positive donors, qnrB4, blaCTX-M-14 and blaSHV-12 genes can be transferred to recipient together. Mutations in GyrA and/or ParC were observed among the four PMQR-positive isolates. There were no mutations in the target genes among the transconjugans and the recipient. Conclusion Our study demonstrated that the clinical isolates ofA. baumannii were positive for qnrB gene. The coexistence and co-transfer of qnr and bla genes may lead to the emergence and spread of multidrug-resistant pathogens.