文献类型：期刊文献

英文题名：Cross-linkage urease nanoparticles: a high-efficiency signal-generation tag for portable pH meter-based electrochemical immunoassay of lipocalin-2 protein diagnostics

作者：Sun, Ai-Li[1];Qi, Qing-An[2];Zhi, Li-Juan[3]

第一作者：孙爱丽

通讯作者：Qi, QA[1]

机构：[1]Xinxiang Univ, Dept Pharm, Key Lab Nanocarbon Modified Film Technol Engn Hen, Xinxiang 453000, Henan, Peoples R China;[2]Hosp Eighty Third Army, Dept Gen Surg, Xinxiang 453000, Henan, Peoples R China;[3]Xinxiang Univ, Dept Chem & Chem Engn, Xinxiang 453000, Henan, Peoples R China

第一机构：新乡学院

通讯机构：[1]corresponding author), Hosp Eighty Third Army, Dept Gen Surg, Xinxiang 453000, Henan, Peoples R China.

年份：2020

卷号：187

期号：9

外文期刊名：MICROCHIMICA ACTA

收录：;Scopus(收录号：2-s2.0-85088963371);WOS:【SCI-EXPANDED(收录号:WOS:000560820500002)】；

基金：This research was financially supported by the National Natural Science Foundation of China (51802275), the Key Scientific and Technological Research Project of Henan Province (202102310298), and the Research Fund for the Technological Innovative Scholar of Xinxiang University, China.

语种：英文

外文关键词：Electrochemical immunoassay; pH measurement; Cross-linkage urease nanoparticles; Signal-transduction tags; Enzymatic hydrolysis

摘要：An innovative signal-transduction tag based on cross-linked urease nanoparticles (CLENP) was designed for the development of a pH meter-based immunoassay of lipocalin-2 (LCN2). The CLENP was synthesized with a typical desolvation method using ethanol as desolvation agent, followed by functionalization with polyaspartic acid. The carboxylated CLENP were used as the signal-generation tags for the labelling of secondary antibodies via the carbodiimide coupling. Upon target LCN2 introduction, a sandwich-type immune reaction was performed between capture antibody-coated plate and the labeled secondary antibody on the CLENP. The conjugated CLENP in the microplate hydrolyzed urea into ammonia (NH4+) and carbonate (CO32-), resulting in the pH change of solution, which was determined with a handheld pH meter. The pH variation was proportional to target concentration in the sample. By monitoring the pH variation of the urea solution, the level of LCN2 at a concentration as low as 5.2 pg mL(-1) was evaluated. The pH meter-based electrochemical immunoassay can be utilized for mass production of miniaturized lab-on-a-chip devices with handheld pH meter, thereby opening new opportunities for protein diagnostics and biosecurity.