文献类型：期刊文献

英文题名：Establishment of a multiplex RT-PCR assay for identification of atmospheric virus contamination in pig farms

作者：Li, Han[1];Wei, Xiaobing[1];Zhang, Xiulin[1];Xu, Hao[3];Zhao, Xuesong[3];Zhou, Shaofeng[4];Huang, Shaobin[3];Liu, Xingyou[2]

第一作者：Li, Han

通讯作者：Li, H[1];Liu, XY[2]

机构：[1]Henan Inst Sci & Technol, Xinxiang 453003, Henan, Peoples R China;[2]Xinxiang Univ, Xinxiang 453003, Henan, Peoples R China;[3]South China Univ Technol, Sch Environm & Energy, Guangzhou 510006, Guangdong, Peoples R China;[4]Guangdong Univ Technol, Sch Environm Sci & Engn, Guangzhou 510006, Guangdong, Peoples R China

第一机构：Henan Inst Sci & Technol, Xinxiang 453003, Henan, Peoples R China

通讯机构：[1]corresponding author), Henan Inst Sci & Technol, Xinxiang 453003, Henan, Peoples R China;[2]corresponding author), Xinxiang Univ, Xinxiang 453003, Henan, Peoples R China.|[11071]新乡学院;

年份：2019

卷号：253

起止页码：358-364

外文期刊名：ENVIRONMENTAL POLLUTION

收录：;EI(收录号：20192907205232);Scopus(收录号：2-s2.0-85068976884);WOS:【SCI-EXPANDED(收录号:WOS:000483406700038)】；

基金：This research was financially supported by the National Natural Science Foundation of China (grant nos. 51708184, and U1701243), the Post-doctoral Science Foundation of China (2018M632784) and the Modern Agriculture Industry Technical System of Henan Province, China (S2012-06-G02).

语种：英文

外文关键词：Monitoring method; Atmospheric environment; Pathogenic microorganism; Multiplex RT-PCR assay

摘要：Spread of pathogens in pig farms not only causes transfection of diseases to other pigs or even farmers working in the farms, but also induces pollution to the living atmospheric environment of the residents around the farm. Therefore, it is necessary to establish a rapid and simple monitoring method. In this study, full genome sequences of common viruses were analyzed in pig farms, in combination with the design of primers, optimization of the reaction parameters, so as to establish a multiplex RT-PCR assay for the identification of classical swine fever virus (CSFV), Japanese encephalitis virus (JEV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus Type 2 (PCV-2), porcine pseudorabies virus (PRV) and porcine parvovirus virus (PPV), which are common in pig farms. This method has a minimal detectable concentration of 10(-3) ng/mu L, which is highly specific. Furthermore, multiplex RT-PCR was applied to examine air samples from 4 pig farms located in different cities of China. The results were in line with those obtained by single PCR. Therefore, this study can be expected to provide essential technique support for the early warning mechanism as well as disease prevention and control system against the major viruses. (C) 2019 Elsevier Ltd. All rights reserved.