文献类型：期刊文献

英文题名：Protective efficacy against Chlamydophila psittaci by oral immunization based on transgenic rice expressing MOMP in mice

作者：Zhang, Xiu-Xiang[1,2];Yu, Hui[2];Wang, Xiao-Hu[3];Li, Xiu-Zhen[4];Zhu, Yan-Ping[5];Li, Hao-Xin[4];Luo, Sheng-Jun[3];Yuan, Zi-Guo[4]

第一作者：Zhang, Xiu-Xiang

通讯作者：Luo, SJ[1]

机构：[1]S China Agr Univ, Coll Agr, Guangzhou 510642, Guangdong, Peoples R China;[2]Jilin Univ, Coll Plant Sci, Div Agr, Mol Biol Lab, Changchun 130062, Jilin Province, Peoples R China;[3]Guangdong Acad Agr Sci, Inst Vet Med, Guangzhou 510640, Guangdong, Peoples R China;[4]S China Agr Univ, Coll Vet Med, Dept Parasitol, Guangzhou 510642, Guangdong, Peoples R China;[5]Xinxiang Univ, Dept Life Sci & Technol, Xinxiang 453000, Henan Province, Peoples R China

第一机构：S China Agr Univ, Coll Agr, Guangzhou 510642, Guangdong, Peoples R China

通讯机构：[1]corresponding author), Guangdong Acad Agr Sci, Inst Vet Med, Guangzhou 510640, Guangdong, Peoples R China.

年份：2013

卷号：31

期号：4

起止页码：698-703

外文期刊名：VACCINE

收录：;Scopus(收录号：2-s2.0-84871939015);WOS:【SCI-EXPANDED(收录号:WOS:000314625900018)】；

基金：This work was supported, in part, by grants from the National Program for High Technology Research (2011AA10A215), the Project of Science and Technology New Star of Zhu Jiang (2011J2200100), the National Natural Science Foundation of China (30901067), the Scientific and Technological Planning Project of Guangdong Province (Grant No. 2010B020307006) and the Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. 20094404120016). The authors gratefully acknowledge Dr. Eskild Petersen and Dr. Tarpey Ian for their help in revising the manuscript.

语种：英文

外文关键词：MOMP; Transgenic rice; Oral immunization; Edible vaccine

摘要：Avian chlamydiosis is caused by Chlamydophila psittaci (Cp. psittaci) and major outer membrane protein (MOMP) of Cp. psittaci is an excellent vaccine candidate. In this study, the MOMP gene was expressed in rice callus by the Agrobacterium tumefaciens vector. The production of protein in transgenic rice seeds was confirmed and quantified by Western-blot and ELISA, the results demonstrating that the antigen was expressed stably. The transgenic rice seeds expressing the MOMP protein were administered by the oral route to BALB/c mice, which developed MOMP-specific serum IgG and fecal IgA antibodies and a splenocyte MOMP-specific proliferative response and significant levels of IFN-gamma, IL-2, IL-4, IL-5 and TGF-beta production. Immunization with MOMP transgenic seeds induced partial protection (50%) against a lethal challenge with the highly virulent Cp. psittaci 6BC strain. Lung function after challenge was less affected compared non-MOMP immunized animals. The results demonstrate the feasibility of using transgenic rice seeds as an oral vaccine to generate protective immunity and reduce the lung lesions in mice against virulent Cp. psittaci 6BC strain. This finding has implications for further development of an oral vaccine against avian chlamydiosis. (C) 2012 Elsevier Ltd. All rights reserved.