文献类型：期刊文献

英文题名：Up-regulated expression of PD-1 and its ligands during acute Classical Swine Fever virus infection in swine

作者：Yue, Feng[1];Zhu, Yan-Ping[1];Zhang, Yan-Fang[1];Sun, Guo-Peng[1];Yang, Yuan[1,2];Guo, Dong-Guang[1];Wang, Ai-Guo[1,2];Li, Bo-Wen[1,2];Yin, Mei[2];Cheng, An-Chun[3];Wang, Ming-Shu[3];Wang, Xuan-Nian[1,2]

第一作者：岳锋

通讯作者：Wang, MS[1]

机构：[1]Xinxiang Univ, Dept Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China;[2]Henan Ist Sci & Technol, Coll Vet & Anim Sci, Xinxiang 453003, Henan, Peoples R China;[3]Sichuan Agr Univ, Inst Prevent Vet Med, Chengdu 611130, Sichuan, Peoples R China

第一机构：新乡学院生命科学技术学院

通讯机构：[1]corresponding author), Sichuan Agr Univ, Inst Prevent Vet Med, Chengdu 611130, Sichuan, Peoples R China.

年份：2014

卷号：97

期号：2

起止页码：251-256

外文期刊名：RESEARCH IN VETERINARY SCIENCE

收录：;Scopus(收录号：2-s2.0-84924954007);WOS:【SCI-EXPANDED(收录号:WOS:000345809300016)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (No. 31272539; No. 31201877).

语种：英文

外文关键词：Classical swine fever virus; Programmed death-1; Ligand; Peripheral blood mononucleur cells; Swine

摘要：In order to investigate the relationship between the PD-1 pathway and impairment of immune responses with the CSFV infection, the mRNA expression of PD-1 and its ligands were evaluated by quantitative polymerase chain reaction (qPCR) during artificial CSFV infection. Simultaneously, expression of IL-2 and IL-10 mRNA were detected. The T cell proliferation and CSFV load in plasma were also measured. Results showed that the expression of PD-1 and its ligands mRNA were significantly increased (p < 0.01) in PBMC from 3 to 7 days post infection (dpi). Meanwhile the level of IL-10 was up-regulated (p < 0.01). The IL-2 mRNA was not obviously changed but it is significantly increased from 14 dpi. The T cell proliferation was notably decreased at 7 dpi. The CSFV load was also increased in plasma. Overall, our results suggest that the expression of PD-1 and its ligands were up-regulated and probably correlated with immune inhibition during acute CSFV infection. (C) 2014 Elsevier Ltd. All rights reserved.