文献类型：期刊文献

中文题名：紫外诱变选育米曲霉氨肽酶高产菌株

英文题名：Breeding of Aspergillus oryzae Aminopeptidase-producing Strains by Ultraviolet Induced Mutation

作者：李鹏[1,2];张艳芳[1];王军[1,2];韩玉婷[1];王选年[1]

第一作者：李鹏

机构：[1]新乡学院生命科学与技术学院生物技术研究中心;[2]郑州大学生命科学学院

第一机构：新乡学院生命科学技术学院

年份：2017

卷号：42

期号：1

起止页码：61-64

中文期刊名：中国调味品

外文期刊名：China Condiment

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：河南省教育厅自然科学研究项目(2011C550002)

语种：中文

中文关键词：米曲霉;紫外诱变;氨肽酶

外文关键词：Aspergillus oryzae ; ultraviolet induced mutation; aminopeptidase

摘要：以米曲霉CICC2066菌株为出发菌株进行紫外诱变,经过多次筛选后得到3株氨肽酶活力显著提高且遗传性较稳定的诱变株UY-15,UY-17和UY-20。将3株诱变株与出发菌株进行发酵实验性能比较,结果表明:米曲霉UY-15,UY-17和UY-20培养42h后制曲中氨肽酶活力分别是出发菌株的1.24,1.38,1.54倍,且UY-20中性蛋白酶活力较出发菌株提高了7%。3株诱变株的孢子量较出发菌株具有优势。
One strain of Aspergillus oryzae CICC2066 is screened by UV induced mutagenesis. With screeningfor several times, three mutants UY-15, UY-17 and UY-20 with higher, stable aminopeptidase activity are obtained. Compared with CICC2066 on fermentation performance, the arninopeptidase activity of mutants UY-15, UY-17 and UY-20 of culturing for 42 h is 1.24, 1.38,1.54 times that of the starting strain. The neutral protease activity of UY-20 is increased by 7% than that of the starting strain. The number of spores of three induced strains is higher than that of CICC2066.