文献类型：期刊文献

中文题名：金银花不同器官三种活性成分含量及其抗氧化活性

英文题名：Content and Antioxidant Activity of Three Active Components in Different Organs of Lonicerae japonicae Thunb.

作者：徐小博[1];徐萍[1];毛雪飞[1];李俊峰[1];任敏[1]

第一作者：徐小博

机构：[1]新乡学院生命科学技术学院

第一机构：新乡学院生命科学技术学院

年份：2018

卷号：39

期号：13

起止页码：41-45

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：河南省科技攻关项目(152102310018);新乡学院博士科研启动项目(1366020055)

语种：中文

中文关键词：金银花;不同器官;绿原酸;木犀草苷;咖啡酸;高效液相色谱法;抗氧化活性

外文关键词：Lonicerae japonicae Thunb.;different organs;chlorogenic acid;galuteolin;caffeic acid;HPLC;antioxidant activity

摘要：以封丘金银花为材料,利用高效液相色谱法建立同时测定金银花不同器官(花、叶、茎)中绿原酸、咖啡酸、木犀草苷3种活性成分含量的检测方法。并以清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基和ABTS^+·自由基的能力为评价指标,对金银花不同器官的抗氧化活性进行比较。结果表明,建立的高效液相色谱方法实现了对3种活性成分的分离,且线性关系良好(R^2≥0.9997),3个器官中3种活性成分的平均回收率在89.45%~102.35%之间。该方法简便、准确,可靠性强。含量测定结果为:绿原酸在花中含量最高,为27.22 mg·g^(-1),其次是茎(19.68 mg·g^(-1))和叶(12.80 mg·g^(-1));木犀草苷在茎中含量最高,为2.31 mg·g^(-1),其次是叶(1.66 mg·g^(-1))和花(1.07 mg·g^(-1));咖啡酸在花、叶和茎的含量分别是1.18、1.05、0.93 mg·g^(-1)。3个器官中,以花的提取物抗氧化能力相对较强,清除DPPH和ABTS^+·自由基的IC_(50)分别为0.07 mg·m L^(-1)和0.05 mg·m L^(-1),茎和叶具有一定的抗氧化活性,两种评价方法均表明,抗氧化能力大小依次是花>茎>叶。
Lonicerae japonicae Thunb.was used as material to establish the high performance liquid chromatography （HPLC） method for simuhaneously determining the content of chlorogenic acid, caffeic acid and galuteolin in different organs （ flowers, leaves and stems）of Lonicerae japonicae Thunb..The antioxidant activities of extracts from different organs of Lonicerae japonica Thunb.were evaluated through DPPH· and ABTS＋· free radical scavenging ability. Results showed that, three active components were separated by the HPLC method with a good linear relationship （R2 ≥ 0.9997 ） and average recovery rates ranged from 89.45% to 102.35% .Therefore, the method was simple, accurate and highly reliable. The content of chlorogenic acid in the flowers was 27.22 mg·g-1 ,followed by the stems（ 19.68 mg·g-1 ）and the leaves（ 12.80 mg·g-1 ）.The content of luteolin in the stems was the highest,which was 2.31 mg·g-1 ,followed by leaves（ 1.66 mg·g-1） and flowers（ 1.07 mg·g-1 ）.The contents of caffeic acid in the flowers,leaves and stems were 1.18,1.05,0.93 mg·g-1, respectively.The extracts from flowers showed the highest antioxidant activity to DPPH· and ABTS ＋ · among three organs with IC50 0.07 mg·mL-1 and 0.05 mg·mL-1 , and the extracts of stems and leaves also showed antioxidant capacity to some extent.Antioxidant capacity order was flowers 〉 stems 〉 leaves.