文献类型：期刊文献

英文题名：Development of a peptide-based immunochromatographic strip for differentiation of serotype O Foot-and-mouth disease virus-infected pigs from vaccinated pigs

作者：Yang, Suzhen[1,2];Yang, Jifei[1];Zhang, Gaiping[1];Qiao, Songlin[1];Wang, Xuannian[3];Zhao, Dong[1];Li, Xuewu[1];Deng, Ruiguang[1];Zhi, Aimin[1];You, Leiming[1];Chai, Sujun[1];Teng, Man[1]

第一作者：Yang, Suzhen

通讯作者：Zhang, GP[1]

机构：[1]Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China;[2]Henan Agr Univ, Coll Vet Med, Zhengzhou, Peoples R China;[3]Xinxiang Univ, Xinxiang, Peoples R China

第一机构：Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

通讯机构：[1]corresponding author), Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China.

年份：2010

卷号：22

期号：3

起止页码：412-415

外文期刊名：JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION

收录：;Scopus(收录号：2-s2.0-77952497015);WOS:【SCI-EXPANDED(收录号:WOS:000278030100011)】；

基金：The study was supported by the Chinese National High Technology Research, Development Program (grant 2007AA100606), National Natural Science Foundation of China (30730068), and Major State Basic Research Development Program (2005CB523200). The authors thank Dr. Norman A. Gregson for editorial assistance. Suzhen Yang and Jifei Yang both contributed equally to this work.

语种：英文

外文关键词：Foot-and-mouth disease; immunochromatographic strip; nonstructural protein; peptide

摘要：An immunochromatographic strip for discriminating Foot-and-mouth disease virus (FMDV) infected from vaccinated pigs was developed based on synthetic peptide. Five peptides designed from the amino acid sequences of nonstructural proteins (NSP) of FMDV were synthesized, and pep5 located in NSP 3B reacted strongly with serum from FMDV-infected pigs but did not react with serum samples from healthy vaccinated pigs. An immunochromatographic strip was developed by using colloidal gold labeled with pep5 as the detector. Staphylococcal protein A and rabbit against peptide-conjugated ovalbumin antibody immunoglobulin G were blotted on the nitrocellulose membrane for the test and control lines. In comparison with 2 commercial NSP enzyme-linked immunosorbent assays, the peptide-based strip showed good specificity and sensitivity. The apparent agreements of this new assay with Ceditest (R) ELISA and UBI (R) ELISA were 98.59% and 96.63%, respectively. These results indicate that the strip can be adequately used to discriminate FMDV-infected animals from vaccinated animals.