详细信息
新乡市某猪场猪瘟病毒的鉴别检测
Identifiction and Detection of Classical Swine Fever Virus in A Pig Farm of Xinxiang
文献类型:期刊文献
中文题名:新乡市某猪场猪瘟病毒的鉴别检测
英文题名:Identifiction and Detection of Classical Swine Fever Virus in A Pig Farm of Xinxiang
作者:孔令芸[1];陈玲丽[1];李冲[1];孙相和[1];李鹏[2];王选年[2];银梅[1]
第一作者:孔令芸
机构:[1]河南科技学院动物科学学院;[2]新乡学院生命科学与技术系
第一机构:河南科技学院动物科学学院,河南新乡453003
年份:2015
卷号:36
期号:3
起止页码:120-123
中文期刊名:动物医学进展
外文期刊名:Progress in Veterinary Medicine
收录:CSTPCD;;北大核心:【北大核心2014】;
基金:国家自然科学基金项目(31201877)
语种:中文
中文关键词:猪瘟病毒;免疫细胞化学;反转录-聚合酶链反应;序列分析
外文关键词:Classical swine fever virus;cell immunochemistry;RT-PCR;sequence analysis
摘要:新乡市某猪场发生疑似猪瘟病例,为了鉴别其为疫苗毒还是野毒,采用细胞免疫化学方法和RT-PCR,并对其NS5B基因进行序列测定,序列比对并构建进化树,结果表明,猪只为猪瘟野毒感染,且分离的猪瘟病毒与石门(Shimen)株在基因序列上未发生大的变异。
The suspicious cases of classical swine fever occurred in a pig farm of Xinxiang.The virus was i-dentified by cell immunochemistry method and RT-PCR.The results of the sequence alignment and phylo-genetic tree of NS5B gene showed that the pigs were infected with classical swine fever virus field strain, and the gene sequence variation of this isolate was not obvious.
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