文献类型：期刊文献

中文题名：PCV2 Cap蛋白刺激PBMCs中IL-21和IL-17A基因转录水平的变化

英文题名：Transcription Level Changes of IL-21 and IL-17A in PBMCs Stimulated by PCV-2 Cap Protein in vitro

作者：朱艳平[1];刘佳[1,2];何勇[1];岳锋[1];李文明[1,2];郭东光[1];孟广超[1];王选年[1,2]

机构：[1]新乡学院生命科学与基础医学学院动物疫病分子诊断河南省工程实验室,河南新乡453003;[2]郑州大学生命科学技术学院,河南郑州450000

第一机构：新乡学院

年份：2021

期号：4

起止页码：7-11

中文期刊名：湖南农业科学

外文期刊名：Hunan Agricultural Sciences

基金：河南省骨干教师资助计划(2018GGJS154);河南省自然科学基金(182300410086);河南省高等学校重点科研项目(19A230009);新乡学院免疫学精品课程(XJJPZY2015-23);新乡学院2020年课程思政示范课程——免疫学。

语种：中文

中文关键词：猪圆环病毒2型;Cap蛋白;外周血单核细胞;IL-21;IL-17A;PD-L1;转录水平;

外文关键词：PCV-2;Cap protein;PBMCs;IL-21;IL-17A;PD-L1;transcription level

摘要：为了研究猪圆环病毒2型(Porcine circovirus type 2,PCV2)Cap蛋白能否模拟PCV2在体外刺激外周血单核细胞(peripheral blood mononuclear cells,PBMCs),使猪白细胞介素21(interleukin-21,IL-21)和IL-17A基因转录水平发生变化,试验根据IL-21和IL-17A的基因序列,设计引物,利用实时荧光定量PCR检测IL-21和IL-17A基因的转录水平。为进一步分析IL-21和IL-17A转录调节的机制,研究还通过实时荧光定量PCR检测了PD-L1的转录水平。结果表明:PCV2 Cap蛋白和PCV2在体外均能刺激PBMCs,导致IL-21、IL-17A和PD-L1基因的转录水平升高,其中PCV2 Cap蛋白导致的IL-21基因转录水平升高更多,几乎是PCV2刺激时表达量的2倍,IL-17A基因的转录水平基本和PCV2刺激时保持一致;PCV2 Cap蛋白能模拟PCV2刺激PD-L1基因转录水平发生变化,但是与PCV2感染时转录水平变化趋势并不完全一致,PCV2刺激时PD-L1的转录水平更高,预示着PD-L1基因与IL-21和IL-17A基因的转录可能存在一定的调节关系。
In order to determine the role of porcine circovirus type 2(PCV-2)Cap protein as a granular antigen,the transcription level changes of porcine interleukin-21(IL-21)and IL-17A in peripheral blood mononuclear cells(PBMCs)stimulated by PCV-2 and PCV-2 Cap protein in vitro were studied.According to the gene sequences of IL-21 and IL-17A,primers were designed and quantitative real-time PCR was used.To further study the regulation mechanism of IL-21 and IL-17A transcription,quantitative real-time PCR was also used to detect the transcription level of PD-L1.The results showed that both PCV-2 CAP protein and PCV-2 stimulated PBMCs in vitro,resulting in increased transcription levels of IL-21,IL-17A and PD-L1 genes;the transcription level of IL-21 gene induced by PCV-2 Cap protein increased more,almost twice the expression level stimulated by PCV-2,while the transcription level of IL-17A gene basically remained the same as that stimulated by PCV-2.PCV-2 Cap protein can simulate PCV-2 to stimulate changes in the transcription level of PD-L1 gene,butit is not completely consistent with the change trend during PCV-2 infection;when stimulated by PCV-2,the transcription level of PD-L1 is higher,which indicates that there may be a certain regulatory relationship between PD-L1 gene and the transcription of IL-21 and IL-17A genes.