文献类型：期刊文献

英文题名：Efflux pump-mediated benzalkonium chloride resistance in Listeria monocytogenes isolated from retail food

作者：Jiang, Xiaobing[1];Yu, Tao[2];Liang, Yu[3];Ji, Shengdong[1];Guo, Xiaowei[3];Ma, Jianmin[1];Zhou, Lijun[4]

第一作者：Jiang, Xiaobing

通讯作者：Zhou, LJ[1]

机构：[1]Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China;[2]Xinxiang Univ, Coll Life Sci & Technol, Xinxiang 453000, Peoples R China;[3]Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453000, Peoples R China;[4]Navy Gen Hosp, Cent Lab, Beijing 100048, Peoples R China

第一机构：Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China

通讯机构：[1]corresponding author), Navy Gen Hosp, Cent Lab, Beijing 100048, Peoples R China.

年份：2016

卷号：217

起止页码：141-145

外文期刊名：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-84945945369);WOS:【SCI-EXPANDED(收录号:WOS:000366767600020)】；

基金：This work was supported by the Key Project of Natural Science of the Education Department of Henan Province, China (15A180006), the Doctoral Scientific Research Foundation of Henan Normal University (01046500152), and the Modern Agricultural Technology Foundation of Henan Province (Z2012-04-02).

语种：英文

外文关键词：Efflux pump; Benzalkonium chloride; Listeria monocytogenes; Resistance

摘要：In this study, efflux pump-mediated benzalkonium chloride (BC) resistance, including plasmid-encoded (Qac protein family and BcrABC) and chromosome-borne efflux pumps, was investigated in Listeria monocytogenes from retail food in China. Among the 59 L. monocytogenes strains, 13 (22.0%) strains were resistant to BC. The PCR results showed that bcrABC was harbored by 2 of 13 BC resistant strains. However, none of the qac genes were detected among the 59 strains. The bcrABC was absent in both of the plasmid cured strains, indicating that this BC resistance determinant was plasmid-encoded in the two bcrABC-positive strains. In the presence of reserpine, most of the bcrABC-negative strains had decreases in the MICs of BC, suggesting the existence of other efflux pumps and their role in BC resistance. After exposed to reserpine, the reduction in BC MICs was observed in the two cured strains, indicating that efflux pumps located on chromosome was also involved in BC resistance. Our findings suggest that food products may act as reservoirs for BC resistant isolates of L monocytogenes and plasmid- and chromosome-encoded efflux pumps could mediate the BC resistance of L monocytogenes, which is especially relevant to the adaption of this organism in food-related environments with frequent BC use. (C) 2015 Elsevier B.V. All rights reserved.