文献类型：期刊文献

中文题名：抗病南瓜种质482-2离体快繁技术研究

英文题名：Studies on the Rapid Propagation in Vitro of Disease Resistant Pumpkin Germplasm 482-2

作者：蔡祖国[1];周会萍[2];赵一鹏[1];杨鹏鸣[1];周俊国[1]

第一作者：蔡祖国

机构：[1]河南科技学院园林学院,河南新乡453003;[2]新乡学院生命科学与技术系,河南新乡453003

第一机构：河南科技学院园林学院,河南新乡453003

年份：2009

期号：1

起止页码：44-46

中文期刊名：北方园艺

外文期刊名：Northern Horticulture

收录：北大核心:【北大核心2008】；

基金：河南科技学院重点科技攻关资助项目(20055047)

语种：中文

中文关键词：南瓜;组织培养;离体再生;离体快繁

外文关键词：Pumpkin; Tissue culture; Regeneration in vitro;Rapid propagation in vitro

摘要：以抗病南瓜种质482-2的种子为起始材料,通过对组培条件下的培养基筛选试验,建立离体快繁技术体系。结果表明:MS+6-BA 1.0 mg/L是南瓜子叶诱导不定芽的适宜培养基,不定芽在MS+6-BA 1.0 mg/L培养基中增殖系数能够达到4.0,在MS+IBA 0.2 mg/L培养基上不定芽生根率达到83.3%。再生小植株经驯化、移栽,成活率可达95%。
Took the seeds of disease resistant pumpkin germplasm 482-2 as the initial material, rapid propagation in vitro was performed by medium selecting. The result showed that the suitable medium for pumpkin adventitious buds regeneration from cotyledon was MS medium supplemented with BA 1.0 mg/L, on the medium of MS supplemented with BA 1.0 mg/L, the multiplication rate was 4.0, and on the rooting medium of MS supplemented with IBA 0.2 mg/L, radication rate reached 83.3%. After acclimatization and transplantation, the regenerated plantlets＇ survival rate was 95%.