文献类型：期刊文献

中文题名：马铃薯促分裂原活化蛋白激酶激酶基因的电子克隆与生物信息学分析

英文题名：Electronic cloning and bioinformatical analysis of MAPKK Gene from Solanum tuberosum

作者：畅丽萍[1];魏琦超[2];周岩[2]

机构：[1]新乡学院土木工程与建筑系;[2]河南科技学院生命科技学院

第一机构：新乡学院土木工程与建筑学院

年份：2012

卷号：39

期号：15

起止页码：159-162

中文期刊名：广东农业科学

外文期刊名：Guangdong Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2011_2012】；

基金：河南省科技厅基础与前沿技术研究计划项目(112300410014)

语种：中文

中文关键词：马铃薯;促分裂原活化蛋白激酶激酶;电子克隆;生物信息学分析

外文关键词：Solarium tuberosum; mitogen-activated protein kinase kinase（MAPKK）; electronic clone; bioinformatics analysis

摘要：摘利用电子克隆获得马铃薯促分裂原活化蛋白激酶激酶基因(MAPKK)的cDNA序列,采用生物信息学方法对该基因及其编码蛋白质的一般理化性质、疏水性、三维结构、亚细胞定位和系统进化关系等方面进行预测和分析。结果表明:马铃薯促分裂原活化蛋白激酶激酶基因的cDNA序列全长1 669 bp,包含1个1 074 bp的ORF,编码357个氨基酸。马铃薯MAPKK为一亲水性的细胞质蛋白,α-螺旋、β-股和无规则卷曲是其主要的二级结构,该酶与同为茄科植物番茄、烟草的MAPKK的亲缘关系最近。
A cDNA sequence of mitogen-activated protein kinase kinase （MAPKK） gene from Solarium tuberosum was cloned by electronic cloning. Some characters of the MAPKK and its encoded protein sequence were predicted and analyzed by the methods of bioinformatics in the following aspects, including the general physical and chemical properties, hydrophobicity, three-dimensional structure, localization sites in cells and phylogenetic relationships. Results showed that the full-length of MAPKK from Solarium tuberosum was 1 669 bp long and it contained a complete ORF （1 074 bp） which encoded 357 amino acids. The MAPKK of Solarium tuberosum was a hydrophilic and cytoplasmic protein. The main secondary structure elements of MAPKK were alpha helix, β-strand and random coil. The phylogenetic analysis showed that the MAPKK of Solarium tuberosum is relatively closer to the MAPKK of Solarium lycopersicum and Nicotiana tabacum, which are all belong to the family of Solananeae.