文献类型：期刊文献

英文题名：Transplantation of Retinal Progenitor Cells from Optic Cup-Like Structures Differentiated from Human Embryonic Stem Cells In Vitro and In Vivo Generation of Retinal Ganglion-Like Cells

作者：Wang, Song-Tao[1,2];Chen, Li-li[3];Zhang, Peng[2];Wang, Xiao-Bing[2];Sun, Yan[2];Ma, Li-Xiang[2];Liu, Qiong[2];Zhou, Guo-Min[2]

第一作者：Wang, Song-Tao

通讯作者：Zhou, GM[1]

机构：[1]Xinxiang Med Univ, Sch Basic Med Sci, Dept Anat, Xinxiang, Peoples R China;[2]Fudan Univ, Shanghai Med Coll, Sch Basic Med Sci, Dept Anat Histol & Embryol, Shanghai 200032, Peoples R China;[3]Xinxiang Univ, Dept Biol Sci & Biotechnol, Xinxiang, Peoples R China

第一机构：Xinxiang Med Univ, Sch Basic Med Sci, Dept Anat, Xinxiang, Peoples R China

通讯机构：[1]corresponding author), Fudan Univ, Shanghai Med Coll, Sch Basic Med Sci, Dept Anat Histol & Embryol, Shanghai 200032, Peoples R China.

年份：2019

卷号：28

期号：4

起止页码：258-267

外文期刊名：STEM CELLS AND DEVELOPMENT

收录：;Scopus(收录号：2-s2.0-85061795201);WOS:【SCI-EXPANDED(收录号:WOS:000463373800002)】；

基金：This study was supported by the National Natural Science Foundation of China (grant nos. 31571238 and 31371083) and the research foundation for doctors of Xinxiang Medical University (grant no. XYBSKYZZ2015010). We gratefully appreciate the help by Professor Su-chun Zhang (Waisman Center, University of Wisconsin) and the support project for the disciplinary group of Physiology and Neuroscience, Xinxiang Medical University.

语种：英文

外文关键词：human embryonic stem cells; optic cup-like structures; transplantation; retinal progenitor cells; retinal ganglion cells

摘要：Human embryonic stem cells (hESCs) have the potential to differentiate along the retinal lineage. We have efficiently differentiated human pluripotent stem cells into optic cup-like structures by using a novel retinal differentiation medium (RDM). The purpose of this study was to determine whether the retinal progenitor cells (RPCs) derived from hESCs can integrate into the host retina and differentiate into retinal ganglion cells (RGCs) in vivo. In this study, hESCs (H9-GFP) were induced to differentiate into optic cup-like structures by using our novel differentiation system. The RPCs extracted from the optic cup-like structures were transplanted into the vitreous cavity of N-methyl-d-aspartic acid-treated mice. Sham-treated eyes received the same amount of RDM. The host retinas were analyzed by triple immunofluorescence on the fourth and fifth weeks after transplantation. The optic cup-like structures were efficiently differentiated from hESCs by using our novel differentiation system in vitro for 6-8 weeks. The RPCs extracted from the optic cup-like structures migrated and integrated into the ganglion cell layer (GCL) of the host retina. Furthermore, the remaining transplanted cells were spread over the GCL and had a complementary distribution with host residual RGCs in the GCL of the mouse retina. Surprisingly, some of the transplanted cells expressed the RGC-specific marker Brn3a. These findings demonstrated that the RPCs derived from hESCs could integrate into the host GCL and differentiate into retinal ganglion-like cells in vivo, suggesting that RPCs can be used as an ideal source in supplying countless RGC and embryonic stem cell-based replacement therapies may be a promising treatment to restore vision in patients with degenerative retinal diseases.